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The formation of a heterodimer between Huntingtin-interacting protein-1 (HIP-­1) and its novel partner HIPPI (HIP-1 protein interactor) through their pseudo death-effector domains (pDEDs) is a key step that recruits caspase-8 and initiates apoptosis. This could be one of the pathways by which apoptosis is increased in Huntington's disease (HD). A construct consisting of the pDED of HIPPI has been cloned and overexpressed as 6NH-tagged protein and purified by Ni-NTA affinity chromatography. Crystals of the pDED of HIPPI were grown in space group P41, with unit-cell parameters a = b = 77.42, c = 33.31 Å and a calculated Matthews coefficient of 1.88 Å3 Da-1 (33% solvent content) with two molecules per asymmetric unit.

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Portable Document Format (PDF) file https://doi.org/10.1107/S1744309106046628/tb5005sup1.pdf
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