research papers
Iripin-5 is the main Ixodes ricinus salivary serpin, which acts as a modulator of host defence mechanisms by impairing neutrophil migration, suppressing nitric oxide production by macrophages and altering complement functions. Iripin-5 influences host immunity and shows high expression in the salivary glands. Here, the crystal structure of Iripin-5 in the most thermodynamically stable state of serpins is described. In the reactive-centre loop, the main substrate-recognition site of Iripin-5 is likely to be represented by Arg342, which implies the targeting of trypsin-like proteases. Furthermore, a computational structural analysis of selected Iripin-5–protease complexes together with interface analysis revealed the most probable residues of Iripin-5 involved in complex formation.
Keywords: serpins; serine protease inhibitors; Iripin-5; X-ray structure; Ixodes ricinus; tick saliva.
Supporting information
Portable Document Format (PDF) file https://doi.org/10.1107/S2059798321007920/rr5207sup1.pdf |
PDB reference: Iripin-5, 7b2t