research papers
Recent years have seen an increase in the development of computational proteins, including symmetric ones. A ninefold-symmetric β-propeller protein named Cake has recently been developed. Here, attempts were made to further engineer this protein into a threefold-symmetric nine-bladed propeller using computational design. Two nine-bladed propeller proteins were designed, named Scone-E and Scone-R. Crystallography, however, revealed the structure of both designs to adopt an eightfold conformation with distorted termini, leading to a pseudo-symmetric protein. One of the proteins could only be crystallized upon the addition of a polyoxometalate, highlighting the usefulness of these molecules as crystallization additives.
Supporting information
Portable Document Format (PDF) file https://doi.org/10.1107/S2059798321005787/ji5022sup1.pdf |
PDB references: Scone-R, 7awy; Scone-E, 7awz; Scone-E + STA, form A, 7ax0; Scone-E + STA, form B, 7ax2