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An automated high-throughput dispenser has been developed for the setup of protein crystallization trials by vapor diffusion or Microbatch methods. The Hydra®-Plus-One is composed of a Hydra®-PP system equipped with a motorized XYZ-platform, 96 precision glass syringes and a single-channel microsolenoid dispenser, which transfers 100nl-50μl of protein solution with an accuracy of > 90% at a speed of 60s per 96 wells. Up to 300μl of premixed cocktails can be aspirated with the 96-syringe-assembly and dispensed into reservoir and droplet wells within 60s. The Hydra®-Plus-One combines high precision, reliability and speed in a cost-effective high-throughput system ideally suited for protein crystallization

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