Crystallization of a Humicola lanuginosa lipase-inhibitor complex with the use of polyethylene glycol monomethyl ether

The fungal Humicola lanuginosa lipase complexed with the inhibitor n-dodecylphosphonate ethyl ester was crystallized in space group P2₁2₁2₁ with pseudotetragonal unit-cell parameters of a = 131.7 (2), b = 131.3 (1), c = 75.4 (1) Å. 92% of X-ray diffraction data to 2.8 Å resolution were collected with a final R_merge = 8.5%. The crystals were grown using a new kind of precipitant - polyethylene glycol monomethyl ether (Peg-mme) of molecular weight 5000.