Crystallization and preliminary X-ray analysis of D-monellin

D-Monellin is a chemically synthesized protein composed of all D-amino acids. It has an amino-acid sequence identical to L-monellin, a natural protein with potent sweetness. Two crystal forms of D-monellin were obtained. Both crystals were grown under conditions similiar to those used to crystallize natural L-monellin. Crystal form I has similar, but not identical, cell parameters to natural L-monellin and diffracts to 2.7 Å resolution. Crystal form II is very different and diffracts to 1.7 Å resolution using synchrotron radiation.