Supporting information
Crystallographic Information File (CIF) https://doi.org/10.1107/S0108270112015296/fa3275sup1.cif | |
Structure factor file (CIF format) https://doi.org/10.1107/S0108270112015296/fa3275Isup2.hkl |
CCDC reference: 879467
Rifampicin (1 mg, 0.001 mmol) was dissolved in a mixture of water (0.02 ml) and ethanol (0.10 ml). From this alcohol–water solution, red [Orange in CIF - please clarify] crystals of (I) suitable for X-ray analysis were obtained.
All H atoms were located in difference electron-density maps. Some water H atoms appeared as peaks of low density and hydrogen-bonding interactions were taken into account for their identification. Atoms H1O1 and H40N were placed in positions determined from a difference electron-density map and refined as riding, with their isotropic displacement parameters freely refined. H atoms of C—H groups and the amide N—H group were placed at idealized positions and refined as riding, with C—H = 0.93–1.00 Å and N—H = 0.88 Å, and with Uiso(H) = 1.2Ueq(C), except for methyl groups, for which Uiso(H) = 1.5Ueq(C). In the water molecules, the O—H and H···H distances were initially restrained to 0.840 (1) and 1.35 (1) Å, respectively, and in the final cycles of refinement a riding model was applied with Uiso(H) = 1.2Ueq(O). The O—H bond lengths in the hydroxy groups (except O1—H1O1) were constrained to 0.84 Å and the Uiso(H) values were constrained to 1.2Ueq(O). In the absence of significant anomalous scattering effects, Friedel pairs were merged as equivalent data. The absolute structure is based on the known absolute configuration of rifampicin.
Data collection: CrysAlis PRO (Agilent, 2010); cell refinement: CrysAlis PRO (Agilent, 2010); data reduction: CrysAlis PRO (Agilent, 2010); program(s) used to solve structure: SIR2004 (Burla et al., 2005); program(s) used to refine structure: SHELXL97 (Sheldrick, 2008); molecular graphics: ORTEP-3 for Windows (Farrugia, 1997) and Mercury (Macrae et al., 2006); software used to prepare material for publication: SHELXL97 (Sheldrick, 2008).
C43H58N4O12·5H2O | F(000) = 1960 |
Mr = 913.01 | Dx = 1.275 Mg m−3 |
Orthorhombic, P212121 | Cu Kα radiation, λ = 1.54178 Å |
Hall symbol: P 2ac 2ab | Cell parameters from 5773 reflections |
a = 13.8506 (6) Å | θ = 2.5–73.6° |
b = 17.3867 (8) Å | µ = 0.82 mm−1 |
c = 19.7476 (8) Å | T = 130 K |
V = 4755.5 (4) Å3 | Plate, red |
Z = 4 | 0.25 × 0.25 × 0.1 mm |
Oxford SuperNova diffractometer | 4805 independent reflections |
Radiation source: Nova Cu X-ray Source | 4531 reflections with I > 2σ(I) |
Mirror monochromator | Rint = 0.029 |
ω scans | θmax = 68.2°, θmin = 6.4° |
Absorption correction: multi-scan (CrysAlis PRO; Agilent, 2010) | h = −16→15 |
Tmin = 0.834, Tmax = 1.000 | k = −20→18 |
13055 measured reflections | l = −23→20 |
Refinement on F2 | Primary atom site location: structure-invariant direct methods |
Least-squares matrix: full | Secondary atom site location: difference Fourier map |
R[F2 > 2σ(F2)] = 0.048 | Hydrogen site location: inferred from neighbouring sites |
wR(F2) = 0.135 | H-atom parameters constrained |
S = 1.06 | w = 1/[σ2(Fo2) + (0.0916P)2 + 1.428P] where P = (Fo2 + 2Fc2)/3 |
4805 reflections | (Δ/σ)max < 0.001 |
597 parameters | Δρmax = 0.44 e Å−3 |
0 restraints | Δρmin = −0.49 e Å−3 |
C43H58N4O12·5H2O | V = 4755.5 (4) Å3 |
Mr = 913.01 | Z = 4 |
Orthorhombic, P212121 | Cu Kα radiation |
a = 13.8506 (6) Å | µ = 0.82 mm−1 |
b = 17.3867 (8) Å | T = 130 K |
c = 19.7476 (8) Å | 0.25 × 0.25 × 0.1 mm |
Oxford SuperNova diffractometer | 4805 independent reflections |
Absorption correction: multi-scan (CrysAlis PRO; Agilent, 2010) | 4531 reflections with I > 2σ(I) |
Tmin = 0.834, Tmax = 1.000 | Rint = 0.029 |
13055 measured reflections |
R[F2 > 2σ(F2)] = 0.048 | 0 restraints |
wR(F2) = 0.135 | H-atom parameters constrained |
S = 1.06 | Δρmax = 0.44 e Å−3 |
4805 reflections | Δρmin = −0.49 e Å−3 |
597 parameters |
Geometry. All e.s.d.'s (except the e.s.d. in the dihedral angle between two l.s. planes) are estimated using the full covariance matrix. The cell e.s.d.'s are taken into account individually in the estimation of e.s.d.'s in distances, angles and torsion angles; correlations between e.s.d.'s in cell parameters are only used when they are defined by crystal symmetry. An approximate (isotropic) treatment of cell e.s.d.'s is used for estimating e.s.d.'s involving l.s. planes. |
Refinement. Refinement of F2 against ALL reflections. The weighted R-factor wR and goodness of fit S are based on F2, conventional R-factors R are based on F, with F set to zero for negative F2. The threshold expression of F2 > σ(F2) is used only for calculating R-factors(gt) etc. and is not relevant to the choice of reflections for refinement. R-factors based on F2 are statistically about twice as large as those based on F, and R-factors based on ALL data will be even larger. |
x | y | z | Uiso*/Ueq | ||
O1 | 0.30015 (16) | 0.75118 (14) | 0.22373 (13) | 0.0373 (5) | |
H1O1 | 0.2452 | 0.7833 | 0.2403 | 0.076 (17)* | |
O4 | 0.15993 (15) | 0.48293 (12) | 0.11122 (12) | 0.0315 (5) | |
H1O4 | 0.1013 | 0.4743 | 0.1191 | 0.038* | |
O6 | −0.13453 (15) | 0.64033 (13) | 0.18474 (12) | 0.0308 (5) | |
O8 | 0.13138 (17) | 0.79511 (13) | 0.24625 (14) | 0.0393 (6) | |
O11 | −0.02229 (15) | 0.47111 (13) | 0.12271 (12) | 0.0331 (5) | |
O12 | −0.19160 (14) | 0.51457 (13) | 0.20226 (11) | 0.0307 (5) | |
O15 | 0.43934 (16) | 0.75649 (13) | 0.12001 (12) | 0.0353 (5) | |
O21 | 0.48009 (17) | 0.59475 (13) | 0.41967 (14) | 0.0378 (5) | |
H21O | 0.4341 | 0.5930 | 0.4473 | 0.057* | |
O23 | 0.31253 (16) | 0.54271 (14) | 0.47347 (12) | 0.0350 (5) | |
H23O | 0.3138 | 0.5285 | 0.5141 | 0.053* | |
O25 | 0.12237 (16) | 0.40545 (13) | 0.39895 (12) | 0.0330 (5) | |
O27 | −0.06585 (19) | 0.50789 (16) | 0.42146 (13) | 0.0438 (6) | |
O35 | 0.0957 (3) | 0.41051 (19) | 0.51087 (15) | 0.0723 (11) | |
N2 | 0.42597 (18) | 0.64209 (15) | 0.17672 (15) | 0.0300 (6) | |
H1N2 | 0.4595 | 0.6062 | 0.1975 | 0.036* | |
N38 | 0.43724 (19) | 0.48898 (15) | 0.15341 (14) | 0.0310 (6) | |
N39 | 0.48980 (18) | 0.42188 (15) | 0.14572 (15) | 0.0322 (6) | |
N40 | 0.62114 (19) | 0.30831 (16) | 0.09569 (17) | 0.0368 (6) | |
H40N | 0.6287 | 0.3242 | 0.0439 | 0.074 (16)* | |
C1 | 0.2627 (2) | 0.68762 (18) | 0.19492 (17) | 0.0308 (6) | |
C2 | 0.3251 (2) | 0.63087 (18) | 0.17121 (16) | 0.0274 (6) | |
C3 | 0.2895 (2) | 0.56034 (18) | 0.14729 (16) | 0.0281 (6) | |
C4 | 0.1896 (2) | 0.55065 (17) | 0.14002 (16) | 0.0266 (6) | |
C5 | 0.0205 (2) | 0.59878 (18) | 0.16089 (16) | 0.0271 (6) | |
C6 | −0.0380 (2) | 0.65786 (18) | 0.18743 (16) | 0.0275 (6) | |
C7 | −0.0065 (2) | 0.72507 (18) | 0.21530 (17) | 0.0311 (7) | |
C8 | 0.0959 (2) | 0.73511 (18) | 0.21786 (17) | 0.0299 (6) | |
C9 | 0.1611 (2) | 0.67629 (17) | 0.19068 (16) | 0.0281 (6) | |
C10 | 0.1247 (2) | 0.60735 (18) | 0.16224 (16) | 0.0263 (6) | |
C11 | −0.0401 (2) | 0.53855 (18) | 0.14348 (16) | 0.0279 (6) | |
C12 | −0.1442 (2) | 0.56402 (18) | 0.15592 (17) | 0.0288 (6) | |
C13 | −0.2049 (2) | 0.5673 (2) | 0.09318 (18) | 0.0352 (7) | |
H13A | −0.2082 | 0.5161 | 0.0726 | 0.053* | |
H13B | −0.1761 | 0.6035 | 0.0609 | 0.053* | |
H13C | −0.2702 | 0.5846 | 0.1049 | 0.053* | |
C14 | −0.0732 (2) | 0.7845 (2) | 0.24442 (19) | 0.0365 (7) | |
H14A | −0.1403 | 0.7693 | 0.2361 | 0.055* | |
H14B | −0.0607 | 0.8343 | 0.2229 | 0.055* | |
H14C | −0.0621 | 0.7888 | 0.2933 | 0.055* | |
C15 | 0.4752 (2) | 0.70370 (18) | 0.15262 (17) | 0.0293 (6) | |
C16 | 0.5826 (2) | 0.70247 (18) | 0.16704 (18) | 0.0317 (7) | |
C17 | 0.6225 (2) | 0.67000 (18) | 0.22144 (18) | 0.0334 (7) | |
H17A | 0.6904 | 0.6761 | 0.2253 | 0.040* | |
C18 | 0.5770 (2) | 0.62645 (19) | 0.27585 (17) | 0.0327 (7) | |
H18A | 0.5113 | 0.6363 | 0.2865 | 0.039* | |
C19 | 0.6246 (2) | 0.57316 (19) | 0.31119 (18) | 0.0341 (7) | |
H19A | 0.6930 | 0.5741 | 0.3074 | 0.041* | |
C20 | 0.5828 (2) | 0.51165 (19) | 0.35637 (18) | 0.0325 (7) | |
H20A | 0.6228 | 0.5091 | 0.3985 | 0.039* | |
C21 | 0.4779 (2) | 0.52739 (19) | 0.37719 (17) | 0.0316 (7) | |
H21A | 0.4406 | 0.5404 | 0.3354 | 0.038* | |
C22 | 0.4270 (2) | 0.45951 (19) | 0.41216 (17) | 0.0329 (7) | |
H22A | 0.4271 | 0.4158 | 0.3793 | 0.039* | |
C23 | 0.3207 (2) | 0.4762 (2) | 0.42976 (17) | 0.0332 (7) | |
H23A | 0.2949 | 0.4307 | 0.4550 | 0.040* | |
C24 | 0.2522 (2) | 0.4926 (2) | 0.37023 (17) | 0.0345 (7) | |
H24A | 0.2636 | 0.5468 | 0.3553 | 0.041* | |
C25 | 0.1468 (2) | 0.48716 (19) | 0.39584 (18) | 0.0327 (7) | |
H25A | 0.1434 | 0.5092 | 0.4426 | 0.039* | |
C26 | 0.0739 (2) | 0.5296 (2) | 0.35056 (18) | 0.0348 (7) | |
H26A | 0.0962 | 0.5243 | 0.3026 | 0.042* | |
C27 | −0.0284 (2) | 0.49624 (19) | 0.35495 (17) | 0.0317 (7) | |
H27A | −0.0245 | 0.4396 | 0.3466 | 0.038* | |
C28 | −0.0948 (2) | 0.53068 (19) | 0.30262 (17) | 0.0314 (7) | |
H28A | −0.1074 | 0.5844 | 0.3034 | 0.038* | |
C29 | −0.1354 (2) | 0.48750 (19) | 0.25586 (16) | 0.0306 (6) | |
H29A | −0.1257 | 0.4335 | 0.2588 | 0.037* | |
C30 | 0.6421 (2) | 0.7413 (2) | 0.1132 (2) | 0.0407 (8) | |
H30A | 0.7104 | 0.7400 | 0.1261 | 0.061* | |
H30B | 0.6212 | 0.7949 | 0.1084 | 0.061* | |
H30C | 0.6334 | 0.7144 | 0.0700 | 0.061* | |
C31 | 0.5934 (3) | 0.4350 (2) | 0.3189 (2) | 0.0418 (8) | |
H31A | 0.5483 | 0.4335 | 0.2807 | 0.063* | |
H31B | 0.5792 | 0.3925 | 0.3499 | 0.063* | |
H31C | 0.6597 | 0.4298 | 0.3020 | 0.063* | |
C32 | 0.4814 (2) | 0.4322 (2) | 0.47501 (18) | 0.0360 (7) | |
H32A | 0.5423 | 0.4080 | 0.4613 | 0.054* | |
H32B | 0.4419 | 0.3947 | 0.4996 | 0.054* | |
H32C | 0.4950 | 0.4762 | 0.5044 | 0.054* | |
C33 | 0.2688 (2) | 0.4403 (2) | 0.30876 (19) | 0.0411 (8) | |
H33A | 0.3312 | 0.4527 | 0.2880 | 0.062* | |
H33B | 0.2171 | 0.4483 | 0.2757 | 0.062* | |
H33C | 0.2689 | 0.3864 | 0.3234 | 0.062* | |
C34 | 0.0757 (3) | 0.6161 (2) | 0.3688 (2) | 0.0495 (10) | |
H34A | 0.0321 | 0.6442 | 0.3386 | 0.074* | |
H34B | 0.1415 | 0.6360 | 0.3637 | 0.074* | |
H34C | 0.0547 | 0.6229 | 0.4158 | 0.074* | |
C35 | 0.0963 (3) | 0.3754 (2) | 0.45912 (18) | 0.0391 (8) | |
C36 | 0.0684 (3) | 0.2929 (2) | 0.4519 (2) | 0.0462 (9) | |
H36A | 0.0427 | 0.2740 | 0.4950 | 0.069* | |
H36B | 0.1253 | 0.2626 | 0.4393 | 0.069* | |
H36C | 0.0190 | 0.2879 | 0.4167 | 0.069* | |
C37 | −0.1416 (3) | 0.4527 (3) | 0.4372 (2) | 0.0585 (12) | |
H37A | −0.1955 | 0.4595 | 0.4057 | 0.088* | |
H37B | −0.1642 | 0.4609 | 0.4836 | 0.088* | |
H37C | −0.1160 | 0.4003 | 0.4329 | 0.088* | |
C38 | 0.3499 (2) | 0.49360 (18) | 0.13115 (17) | 0.0308 (7) | |
H38A | 0.3244 | 0.4533 | 0.1040 | 0.037* | |
C39 | 0.4555 (2) | 0.3627 (2) | 0.0993 (2) | 0.0404 (8) | |
H39B | 0.4601 | 0.3815 | 0.0521 | 0.048* | |
H39A | 0.3871 | 0.3507 | 0.1090 | 0.048* | |
C40 | 0.5166 (2) | 0.2910 (2) | 0.1077 (2) | 0.0422 (9) | |
H40A | 0.5082 | 0.2705 | 0.1541 | 0.051* | |
H40B | 0.4947 | 0.2513 | 0.0753 | 0.051* | |
C41 | 0.6528 (2) | 0.37040 (19) | 0.1427 (2) | 0.0366 (7) | |
H41B | 0.7211 | 0.3835 | 0.1336 | 0.044* | |
H41A | 0.6479 | 0.3520 | 0.1900 | 0.044* | |
C42 | 0.5906 (2) | 0.44131 (19) | 0.13374 (19) | 0.0336 (7) | |
H42A | 0.6113 | 0.4817 | 0.1660 | 0.040* | |
H42B | 0.5983 | 0.4616 | 0.0872 | 0.040* | |
C43 | 0.6816 (3) | 0.2379 (2) | 0.1026 (3) | 0.0476 (9) | |
H43A | 0.6749 | 0.2172 | 0.1486 | 0.071* | |
H43C | 0.7494 | 0.2508 | 0.0941 | 0.071* | |
H43B | 0.6602 | 0.1992 | 0.0698 | 0.071* | |
O4W | 0.9289 (4) | 0.7896 (3) | 0.4567 (3) | 0.1177 (18) | |
H1W4 | 0.9792 | 0.7982 | 0.4342 | 0.141* | |
H2W4 | 0.9080 | 0.7469 | 0.4434 | 0.141* | |
O1W | 0.8404 (2) | 0.6444 (2) | 0.46457 (18) | 0.0648 (8) | |
H1W1 | 0.7824 | 0.6413 | 0.4525 | 0.078* | |
H2W1 | 0.8672 | 0.6034 | 0.4521 | 0.078* | |
O2W | 0.6468 (3) | 0.6578 (3) | 0.47693 (19) | 0.0821 (12) | |
H1W2 | 0.6512 | 0.6581 | 0.5194 | 0.098* | |
H2W2 | 0.6008 | 0.6298 | 0.4642 | 0.098* | |
O3W | 0.60292 (19) | 0.66891 (15) | 0.61782 (14) | 0.0443 (6) | |
H1W3 | 0.6159 | 0.6842 | 0.6572 | 0.053* | |
H2W3 | 0.5826 | 0.6237 | 0.6225 | 0.053* | |
O5W | 0.2631 (3) | 0.7935 (3) | 0.0540 (2) | 0.0970 (15) | |
H1W5 | 0.3145 | 0.7822 | 0.0743 | 0.116* | |
H2W5 | 0.2546 | 0.7581 | 0.0257 | 0.116* |
U11 | U22 | U33 | U12 | U13 | U23 | |
O1 | 0.0213 (10) | 0.0336 (11) | 0.0571 (14) | −0.0064 (9) | 0.0018 (10) | −0.0121 (11) |
O4 | 0.0163 (9) | 0.0312 (11) | 0.0470 (12) | −0.0015 (8) | 0.0009 (9) | −0.0083 (10) |
O6 | 0.0147 (9) | 0.0302 (11) | 0.0475 (12) | 0.0004 (8) | −0.0004 (9) | −0.0016 (10) |
O8 | 0.0240 (10) | 0.0316 (12) | 0.0622 (15) | −0.0036 (10) | 0.0028 (11) | −0.0125 (11) |
O11 | 0.0198 (10) | 0.0295 (11) | 0.0501 (13) | −0.0040 (9) | 0.0032 (9) | −0.0033 (10) |
O12 | 0.0157 (9) | 0.0363 (11) | 0.0402 (11) | −0.0030 (9) | −0.0004 (9) | 0.0024 (9) |
O15 | 0.0243 (10) | 0.0299 (11) | 0.0515 (13) | 0.0011 (9) | −0.0007 (10) | 0.0024 (10) |
O21 | 0.0254 (11) | 0.0313 (11) | 0.0569 (14) | −0.0015 (9) | 0.0047 (11) | −0.0056 (11) |
O23 | 0.0236 (10) | 0.0409 (12) | 0.0406 (12) | −0.0018 (10) | −0.0029 (10) | 0.0012 (10) |
O25 | 0.0247 (10) | 0.0343 (11) | 0.0399 (11) | −0.0034 (9) | −0.0019 (9) | 0.0041 (10) |
O27 | 0.0369 (13) | 0.0519 (15) | 0.0426 (13) | −0.0017 (12) | 0.0017 (11) | 0.0019 (12) |
O35 | 0.119 (3) | 0.0573 (18) | 0.0405 (15) | −0.031 (2) | −0.0021 (18) | 0.0029 (14) |
N2 | 0.0154 (11) | 0.0280 (13) | 0.0465 (15) | 0.0000 (10) | −0.0017 (11) | 0.0013 (11) |
N38 | 0.0204 (12) | 0.0273 (13) | 0.0454 (15) | 0.0035 (10) | 0.0003 (11) | −0.0002 (11) |
N39 | 0.0176 (12) | 0.0269 (13) | 0.0519 (16) | 0.0011 (10) | −0.0001 (12) | −0.0005 (12) |
N40 | 0.0199 (13) | 0.0301 (13) | 0.0604 (18) | 0.0017 (11) | 0.0005 (12) | −0.0046 (13) |
C1 | 0.0217 (14) | 0.0276 (15) | 0.0432 (17) | −0.0035 (12) | 0.0006 (13) | −0.0005 (13) |
C2 | 0.0158 (13) | 0.0296 (15) | 0.0369 (15) | −0.0024 (12) | −0.0002 (12) | 0.0021 (12) |
C3 | 0.0189 (13) | 0.0288 (15) | 0.0367 (15) | −0.0006 (12) | 0.0000 (12) | 0.0001 (13) |
C4 | 0.0195 (13) | 0.0254 (14) | 0.0348 (15) | −0.0015 (11) | −0.0010 (12) | 0.0007 (12) |
C5 | 0.0154 (13) | 0.0285 (15) | 0.0375 (15) | −0.0011 (12) | 0.0002 (12) | 0.0000 (12) |
C6 | 0.0164 (13) | 0.0297 (15) | 0.0364 (15) | −0.0019 (12) | −0.0006 (12) | 0.0036 (13) |
C7 | 0.0196 (14) | 0.0295 (15) | 0.0441 (17) | 0.0026 (12) | 0.0018 (13) | 0.0013 (13) |
C8 | 0.0205 (14) | 0.0270 (15) | 0.0421 (16) | −0.0010 (12) | 0.0006 (13) | 0.0010 (13) |
C9 | 0.0186 (14) | 0.0275 (15) | 0.0382 (15) | −0.0013 (12) | −0.0007 (12) | 0.0008 (13) |
C10 | 0.0173 (13) | 0.0267 (14) | 0.0348 (15) | −0.0030 (11) | −0.0002 (11) | 0.0018 (12) |
C11 | 0.0179 (13) | 0.0297 (15) | 0.0362 (15) | 0.0003 (11) | −0.0001 (12) | 0.0003 (13) |
C12 | 0.0170 (13) | 0.0288 (15) | 0.0404 (16) | −0.0028 (12) | 0.0010 (12) | 0.0001 (13) |
C13 | 0.0226 (14) | 0.0390 (17) | 0.0439 (17) | −0.0005 (13) | −0.0025 (13) | 0.0037 (15) |
C14 | 0.0216 (14) | 0.0335 (16) | 0.0544 (19) | 0.0029 (13) | 0.0025 (14) | −0.0053 (15) |
C15 | 0.0188 (14) | 0.0271 (14) | 0.0421 (16) | −0.0004 (12) | 0.0014 (12) | −0.0019 (13) |
C16 | 0.0168 (14) | 0.0267 (15) | 0.0515 (18) | −0.0012 (12) | 0.0030 (13) | −0.0017 (14) |
C17 | 0.0174 (14) | 0.0291 (15) | 0.0538 (19) | −0.0013 (12) | 0.0004 (14) | −0.0023 (14) |
C18 | 0.0185 (13) | 0.0323 (16) | 0.0472 (17) | −0.0007 (12) | 0.0002 (13) | −0.0030 (14) |
C19 | 0.0162 (13) | 0.0364 (17) | 0.0495 (18) | 0.0007 (12) | −0.0012 (13) | −0.0011 (15) |
C20 | 0.0228 (14) | 0.0318 (16) | 0.0428 (17) | 0.0035 (12) | −0.0029 (14) | −0.0010 (14) |
C21 | 0.0213 (14) | 0.0316 (15) | 0.0418 (17) | −0.0003 (13) | −0.0045 (13) | 0.0012 (13) |
C22 | 0.0272 (15) | 0.0329 (16) | 0.0385 (16) | −0.0019 (13) | −0.0041 (13) | 0.0011 (13) |
C23 | 0.0251 (15) | 0.0358 (16) | 0.0387 (16) | −0.0038 (13) | −0.0039 (13) | 0.0050 (14) |
C24 | 0.0206 (13) | 0.0422 (18) | 0.0407 (17) | −0.0062 (13) | −0.0027 (13) | 0.0060 (15) |
C25 | 0.0236 (15) | 0.0325 (16) | 0.0418 (17) | −0.0051 (13) | −0.0042 (13) | 0.0068 (13) |
C26 | 0.0245 (15) | 0.0358 (17) | 0.0442 (17) | −0.0067 (13) | −0.0071 (14) | 0.0077 (15) |
C27 | 0.0226 (14) | 0.0340 (16) | 0.0385 (16) | −0.0016 (13) | −0.0018 (13) | 0.0030 (13) |
C28 | 0.0186 (13) | 0.0337 (15) | 0.0419 (16) | 0.0012 (12) | −0.0003 (13) | 0.0017 (14) |
C29 | 0.0168 (12) | 0.0339 (16) | 0.0412 (16) | −0.0016 (12) | 0.0009 (12) | 0.0026 (13) |
C30 | 0.0223 (15) | 0.0352 (17) | 0.065 (2) | −0.0023 (13) | 0.0074 (16) | 0.0081 (16) |
C31 | 0.0369 (18) | 0.0357 (17) | 0.053 (2) | 0.0047 (15) | 0.0097 (16) | −0.0005 (16) |
C32 | 0.0275 (15) | 0.0367 (17) | 0.0438 (17) | 0.0045 (14) | −0.0050 (14) | 0.0031 (14) |
C33 | 0.0268 (15) | 0.054 (2) | 0.0425 (18) | −0.0081 (15) | −0.0044 (14) | 0.0031 (17) |
C34 | 0.0403 (19) | 0.0369 (19) | 0.071 (3) | −0.0080 (16) | −0.017 (2) | 0.0090 (18) |
C35 | 0.0370 (18) | 0.0456 (19) | 0.0348 (17) | −0.0057 (16) | −0.0056 (14) | 0.0060 (15) |
C36 | 0.050 (2) | 0.0386 (19) | 0.050 (2) | −0.0062 (18) | 0.0048 (18) | 0.0043 (16) |
C37 | 0.0349 (19) | 0.085 (3) | 0.056 (2) | −0.008 (2) | 0.0096 (18) | 0.012 (2) |
C38 | 0.0216 (14) | 0.0278 (15) | 0.0430 (17) | −0.0026 (12) | 0.0029 (13) | −0.0008 (13) |
C39 | 0.0215 (15) | 0.0320 (16) | 0.068 (2) | 0.0013 (13) | −0.0032 (15) | −0.0101 (16) |
C40 | 0.0190 (15) | 0.0300 (16) | 0.077 (3) | −0.0023 (13) | 0.0005 (16) | −0.0050 (17) |
C41 | 0.0202 (14) | 0.0330 (16) | 0.057 (2) | 0.0020 (13) | −0.0025 (14) | −0.0046 (15) |
C42 | 0.0189 (13) | 0.0276 (15) | 0.0542 (19) | −0.0006 (12) | −0.0004 (14) | −0.0031 (14) |
C43 | 0.0264 (16) | 0.0315 (17) | 0.085 (3) | 0.0056 (15) | −0.0023 (18) | −0.0045 (18) |
O4W | 0.110 (4) | 0.111 (4) | 0.132 (4) | −0.029 (3) | 0.039 (4) | −0.017 (3) |
O1W | 0.0480 (17) | 0.071 (2) | 0.075 (2) | −0.0070 (16) | −0.0088 (16) | −0.0003 (17) |
O2W | 0.0504 (19) | 0.124 (3) | 0.072 (2) | −0.031 (2) | −0.0049 (17) | −0.018 (2) |
O3W | 0.0395 (13) | 0.0356 (13) | 0.0577 (15) | −0.0098 (11) | −0.0009 (12) | −0.0024 (11) |
O5W | 0.072 (2) | 0.130 (4) | 0.090 (3) | 0.038 (3) | −0.012 (2) | 0.017 (3) |
O1—C1 | 1.347 (4) | C21—H21A | 1.0000 |
O1—H1O1 | 1.0000 | C22—C32 | 1.528 (5) |
O4—C4 | 1.370 (4) | C22—C23 | 1.540 (4) |
O4—H1O4 | 0.8400 | C22—H22A | 1.0000 |
O6—C6 | 1.373 (4) | C23—C24 | 1.538 (4) |
O6—C12 | 1.450 (4) | C23—H23A | 1.0000 |
O8—C8 | 1.282 (4) | C24—C33 | 1.534 (5) |
O11—C11 | 1.266 (4) | C24—C25 | 1.548 (4) |
O12—C29 | 1.395 (4) | C24—H24A | 1.0000 |
O12—C12 | 1.417 (4) | C25—C26 | 1.537 (4) |
O15—C15 | 1.226 (4) | C25—H25A | 1.0000 |
O21—C21 | 1.441 (4) | C26—C27 | 1.534 (4) |
O21—H21O | 0.8400 | C26—C34 | 1.547 (5) |
O23—C23 | 1.448 (4) | C26—H26A | 1.0000 |
O23—H23O | 0.8400 | C27—C28 | 1.507 (4) |
O25—C35 | 1.347 (4) | C27—H27A | 1.0000 |
O25—C25 | 1.462 (4) | C28—C29 | 1.317 (5) |
O27—C27 | 1.426 (4) | C28—H28A | 0.9500 |
O27—C37 | 1.455 (5) | C29—H29A | 0.9500 |
O35—C35 | 1.190 (5) | C30—H30A | 0.9800 |
N2—C15 | 1.356 (4) | C30—H30B | 0.9800 |
N2—C2 | 1.414 (4) | C30—H30C | 0.9800 |
N2—H1N2 | 0.8800 | C31—H31A | 0.9800 |
N38—C38 | 1.290 (4) | C31—H31B | 0.9800 |
N38—N39 | 1.383 (4) | C31—H31C | 0.9800 |
N39—C42 | 1.456 (4) | C32—H32A | 0.9800 |
N39—C39 | 1.457 (4) | C32—H32B | 0.9800 |
N40—C43 | 1.490 (4) | C32—H32C | 0.9800 |
N40—C41 | 1.490 (4) | C33—H33A | 0.9800 |
N40—C40 | 1.498 (4) | C33—H33B | 0.9800 |
N40—H40N | 1.0639 | C33—H33C | 0.9800 |
C1—C2 | 1.393 (5) | C34—H34A | 0.9800 |
C1—C9 | 1.423 (4) | C34—H34B | 0.9800 |
C2—C3 | 1.404 (5) | C34—H34C | 0.9800 |
C3—C4 | 1.402 (4) | C35—C36 | 1.493 (5) |
C3—C38 | 1.465 (4) | C36—H36A | 0.9800 |
C4—C10 | 1.404 (4) | C36—H36B | 0.9800 |
C5—C11 | 1.385 (4) | C36—H36C | 0.9800 |
C5—C6 | 1.409 (4) | C37—H37A | 0.9800 |
C5—C10 | 1.451 (4) | C37—H37B | 0.9800 |
C6—C7 | 1.363 (5) | C37—H37C | 0.9800 |
C7—C8 | 1.430 (4) | C38—H38A | 0.9500 |
C7—C14 | 1.501 (4) | C39—C40 | 1.515 (5) |
C8—C9 | 1.467 (4) | C39—H39B | 0.9900 |
C9—C10 | 1.417 (4) | C39—H39A | 0.9900 |
C11—C12 | 1.528 (4) | C40—H40A | 0.9900 |
C12—C13 | 1.498 (5) | C40—H40B | 0.9900 |
C13—H13A | 0.9800 | C41—C42 | 1.514 (4) |
C13—H13B | 0.9800 | C41—H41B | 0.9900 |
C13—H13C | 0.9800 | C41—H41A | 0.9900 |
C14—H14A | 0.9800 | C42—H42A | 0.9900 |
C14—H14B | 0.9800 | C42—H42B | 0.9900 |
C14—H14C | 0.9800 | C43—H43A | 0.9800 |
C15—C16 | 1.514 (4) | C43—H43C | 0.9800 |
C16—C17 | 1.334 (5) | C43—H43B | 0.9800 |
C16—C30 | 1.506 (5) | O4W—H1W4 | 0.8399 |
C17—C18 | 1.458 (5) | O4W—H2W4 | 0.8400 |
C17—H17A | 0.9500 | O1W—H1W1 | 0.8402 |
C18—C19 | 1.334 (5) | O1W—H2W1 | 0.8403 |
C18—H18A | 0.9500 | O2W—H1W2 | 0.8401 |
C19—C20 | 1.508 (5) | O2W—H2W2 | 0.8400 |
C19—H19A | 0.9500 | O3W—H1W3 | 0.8401 |
C20—C31 | 1.532 (5) | O3W—H2W3 | 0.8397 |
C20—C21 | 1.535 (4) | O5W—H1W5 | 0.8402 |
C20—H20A | 1.0000 | O5W—H2W5 | 0.8399 |
C21—C22 | 1.538 (5) | ||
C1—O1—H1O1 | 107.7 | C33—C24—C25 | 111.3 (3) |
C4—O4—H1O4 | 111.5 | C23—C24—C25 | 108.7 (3) |
C6—O6—C12 | 108.0 (2) | C33—C24—H24A | 107.6 |
C29—O12—C12 | 115.9 (2) | C23—C24—H24A | 107.6 |
C21—O21—H21O | 109.5 | C25—C24—H24A | 107.6 |
C23—O23—H23O | 109.5 | O25—C25—C26 | 109.8 (3) |
C35—O25—C25 | 118.4 (3) | O25—C25—C24 | 107.0 (3) |
C27—O27—C37 | 111.4 (3) | C26—C25—C24 | 113.6 (3) |
C15—N2—C2 | 125.4 (3) | O25—C25—H25A | 108.8 |
C15—N2—H1N2 | 117.3 | C26—C25—H25A | 108.8 |
C2—N2—H1N2 | 117.3 | C24—C25—H25A | 108.8 |
C38—N38—N39 | 120.6 (3) | C27—C26—C25 | 113.1 (3) |
N38—N39—C42 | 109.1 (2) | C27—C26—C34 | 111.6 (3) |
N38—N39—C39 | 119.5 (3) | C25—C26—C34 | 108.7 (3) |
C42—N39—C39 | 111.9 (3) | C27—C26—H26A | 107.7 |
C43—N40—C41 | 111.9 (3) | C25—C26—H26A | 107.7 |
C43—N40—C40 | 111.3 (3) | C34—C26—H26A | 107.7 |
C41—N40—C40 | 109.3 (3) | O27—C27—C28 | 110.7 (3) |
C43—N40—H40N | 104.2 | O27—C27—C26 | 109.5 (3) |
C41—N40—H40N | 112.5 | C28—C27—C26 | 112.0 (3) |
C40—N40—H40N | 107.5 | O27—C27—H27A | 108.2 |
O1—C1—C2 | 118.9 (3) | C28—C27—H27A | 108.2 |
O1—C1—C9 | 121.3 (3) | C26—C27—H27A | 108.2 |
C2—C1—C9 | 119.7 (3) | C29—C28—C27 | 121.0 (3) |
C1—C2—C3 | 120.9 (3) | C29—C28—H28A | 119.5 |
C1—C2—N2 | 119.3 (3) | C27—C28—H28A | 119.5 |
C3—C2—N2 | 119.6 (3) | C28—C29—O12 | 125.3 (3) |
C4—C3—C2 | 119.1 (3) | C28—C29—H29A | 117.3 |
C4—C3—C38 | 116.5 (3) | O12—C29—H29A | 117.3 |
C2—C3—C38 | 124.3 (3) | C16—C30—H30A | 109.5 |
O4—C4—C3 | 116.2 (3) | C16—C30—H30B | 109.5 |
O4—C4—C10 | 122.8 (3) | H30A—C30—H30B | 109.5 |
C3—C4—C10 | 121.0 (3) | C16—C30—H30C | 109.5 |
C11—C5—C6 | 107.2 (3) | H30A—C30—H30C | 109.5 |
C11—C5—C10 | 133.2 (3) | H30B—C30—H30C | 109.5 |
C6—C5—C10 | 119.3 (3) | C20—C31—H31A | 109.5 |
C7—C6—O6 | 121.1 (3) | C20—C31—H31B | 109.5 |
C7—C6—C5 | 126.3 (3) | H31A—C31—H31B | 109.5 |
O6—C6—C5 | 112.6 (3) | C20—C31—H31C | 109.5 |
C6—C7—C8 | 115.8 (3) | H31A—C31—H31C | 109.5 |
C6—C7—C14 | 123.3 (3) | H31B—C31—H31C | 109.5 |
C8—C7—C14 | 120.8 (3) | C22—C32—H32A | 109.5 |
O8—C8—C7 | 119.7 (3) | C22—C32—H32B | 109.5 |
O8—C8—C9 | 119.4 (3) | H32A—C32—H32B | 109.5 |
C7—C8—C9 | 120.9 (3) | C22—C32—H32C | 109.5 |
C10—C9—C1 | 119.5 (3) | H32A—C32—H32C | 109.5 |
C10—C9—C8 | 121.0 (3) | H32B—C32—H32C | 109.5 |
C1—C9—C8 | 119.4 (3) | C24—C33—H33A | 109.5 |
C4—C10—C9 | 119.4 (3) | C24—C33—H33B | 109.5 |
C4—C10—C5 | 124.0 (3) | H33A—C33—H33B | 109.5 |
C9—C10—C5 | 116.6 (3) | C24—C33—H33C | 109.5 |
O11—C11—C5 | 131.5 (3) | H33A—C33—H33C | 109.5 |
O11—C11—C12 | 120.3 (3) | H33B—C33—H33C | 109.5 |
C5—C11—C12 | 108.2 (3) | C26—C34—H34A | 109.5 |
O12—C12—O6 | 110.2 (2) | C26—C34—H34B | 109.5 |
O12—C12—C13 | 107.3 (2) | H34A—C34—H34B | 109.5 |
O6—C12—C13 | 109.9 (3) | C26—C34—H34C | 109.5 |
O12—C12—C11 | 111.4 (3) | H34A—C34—H34C | 109.5 |
O6—C12—C11 | 104.0 (2) | H34B—C34—H34C | 109.5 |
C13—C12—C11 | 114.1 (3) | O35—C35—O25 | 124.1 (3) |
C12—C13—H13A | 109.5 | O35—C35—C36 | 124.9 (4) |
C12—C13—H13B | 109.5 | O25—C35—C36 | 110.9 (3) |
H13A—C13—H13B | 109.5 | C35—C36—H36A | 109.5 |
C12—C13—H13C | 109.5 | C35—C36—H36B | 109.5 |
H13A—C13—H13C | 109.5 | H36A—C36—H36B | 109.5 |
H13B—C13—H13C | 109.5 | C35—C36—H36C | 109.5 |
C7—C14—H14A | 109.5 | H36A—C36—H36C | 109.5 |
C7—C14—H14B | 109.5 | H36B—C36—H36C | 109.5 |
H14A—C14—H14B | 109.5 | O27—C37—H37A | 109.5 |
C7—C14—H14C | 109.5 | O27—C37—H37B | 109.5 |
H14A—C14—H14C | 109.5 | H37A—C37—H37B | 109.5 |
H14B—C14—H14C | 109.5 | O27—C37—H37C | 109.5 |
O15—C15—N2 | 124.9 (3) | H37A—C37—H37C | 109.5 |
O15—C15—C16 | 120.5 (3) | H37B—C37—H37C | 109.5 |
N2—C15—C16 | 114.6 (3) | N38—C38—C3 | 120.7 (3) |
C17—C16—C30 | 122.1 (3) | N38—C38—H38A | 119.6 |
C17—C16—C15 | 124.4 (3) | C3—C38—H38A | 119.6 |
C30—C16—C15 | 113.5 (3) | N39—C39—C40 | 109.3 (3) |
C16—C17—C18 | 129.4 (3) | N39—C39—H39B | 109.8 |
C16—C17—H17A | 115.3 | C40—C39—H39B | 109.8 |
C18—C17—H17A | 115.3 | N39—C39—H39A | 109.8 |
C19—C18—C17 | 122.2 (3) | C40—C39—H39A | 109.8 |
C19—C18—H18A | 118.9 | H39B—C39—H39A | 108.3 |
C17—C18—H18A | 118.9 | N40—C40—C39 | 110.9 (3) |
C18—C19—C20 | 127.7 (3) | N40—C40—H40A | 109.5 |
C18—C19—H19A | 116.1 | C39—C40—H40A | 109.5 |
C20—C19—H19A | 116.1 | N40—C40—H40B | 109.5 |
C19—C20—C31 | 107.1 (3) | C39—C40—H40B | 109.5 |
C19—C20—C21 | 113.3 (3) | H40A—C40—H40B | 108.0 |
C31—C20—C21 | 112.1 (3) | N40—C41—C42 | 110.5 (3) |
C19—C20—H20A | 108.1 | N40—C41—H41B | 109.6 |
C31—C20—H20A | 108.1 | C42—C41—H41B | 109.6 |
C21—C20—H20A | 108.1 | N40—C41—H41A | 109.6 |
O21—C21—C20 | 106.3 (2) | C42—C41—H41A | 109.6 |
O21—C21—C22 | 111.8 (3) | H41B—C41—H41A | 108.1 |
C20—C21—C22 | 114.6 (3) | N39—C42—C41 | 109.7 (3) |
O21—C21—H21A | 107.9 | N39—C42—H42A | 109.7 |
C20—C21—H21A | 107.9 | C41—C42—H42A | 109.7 |
C22—C21—H21A | 107.9 | N39—C42—H42B | 109.7 |
C32—C22—C21 | 112.2 (3) | C41—C42—H42B | 109.7 |
C32—C22—C23 | 110.3 (3) | H42A—C42—H42B | 108.2 |
C21—C22—C23 | 113.3 (3) | N40—C43—H43A | 109.5 |
C32—C22—H22A | 106.9 | N40—C43—H43C | 109.5 |
C21—C22—H22A | 106.9 | H43A—C43—H43C | 109.5 |
C23—C22—H22A | 106.9 | N40—C43—H43B | 109.5 |
O23—C23—C24 | 105.0 (3) | H43A—C43—H43B | 109.5 |
O23—C23—C22 | 111.1 (3) | H43C—C43—H43B | 109.5 |
C24—C23—C22 | 116.9 (3) | H1W4—O4W—H2W4 | 106.0 |
O23—C23—H23A | 107.8 | H1W1—O1W—H2W1 | 106.5 |
C24—C23—H23A | 107.8 | H1W2—O2W—H2W2 | 110.9 |
C22—C23—H23A | 107.8 | H1W3—O3W—H2W3 | 105.4 |
C33—C24—C23 | 113.7 (3) | H1W5—O5W—H2W5 | 105.4 |
C38—N38—N39—C42 | 144.6 (3) | C5—C11—C12—C13 | 116.6 (3) |
C38—N38—N39—C39 | 14.1 (5) | C2—N2—C15—O15 | −3.5 (5) |
O1—C1—C2—C3 | 173.3 (3) | C2—N2—C15—C16 | 178.4 (3) |
C9—C1—C2—C3 | −4.3 (5) | O15—C15—C16—C17 | 150.9 (3) |
O1—C1—C2—N2 | −1.3 (5) | N2—C15—C16—C17 | −30.9 (5) |
C9—C1—C2—N2 | −178.9 (3) | O15—C15—C16—C30 | −29.6 (5) |
C15—N2—C2—C1 | −54.6 (5) | N2—C15—C16—C30 | 148.6 (3) |
C15—N2—C2—C3 | 130.8 (3) | C30—C16—C17—C18 | −175.9 (3) |
C1—C2—C3—C4 | 7.4 (5) | C15—C16—C17—C18 | 3.5 (6) |
N2—C2—C3—C4 | −178.0 (3) | C16—C17—C18—C19 | 152.9 (4) |
C1—C2—C3—C38 | −170.4 (3) | C17—C18—C19—C20 | −164.3 (3) |
N2—C2—C3—C38 | 4.2 (5) | C18—C19—C20—C31 | 106.8 (4) |
C2—C3—C4—O4 | 174.8 (3) | C18—C19—C20—C21 | −17.3 (5) |
C38—C3—C4—O4 | −7.3 (4) | C19—C20—C21—O21 | −66.5 (3) |
C2—C3—C4—C10 | −6.1 (5) | C31—C20—C21—O21 | 172.1 (3) |
C38—C3—C4—C10 | 171.8 (3) | C19—C20—C21—C22 | 169.4 (3) |
C12—O6—C6—C7 | −176.9 (3) | C31—C20—C21—C22 | 48.0 (4) |
C12—O6—C6—C5 | 1.2 (4) | O21—C21—C22—C32 | −64.4 (4) |
C11—C5—C6—C7 | 174.8 (3) | C20—C21—C22—C32 | 56.7 (4) |
C10—C5—C6—C7 | 0.1 (5) | O21—C21—C22—C23 | 61.2 (3) |
C11—C5—C6—O6 | −3.2 (4) | C20—C21—C22—C23 | −177.7 (3) |
C10—C5—C6—O6 | −177.9 (3) | C32—C22—C23—O23 | 68.4 (3) |
O6—C6—C7—C8 | 177.3 (3) | C21—C22—C23—O23 | −58.2 (4) |
C5—C6—C7—C8 | −0.5 (5) | C32—C22—C23—C24 | −171.1 (3) |
O6—C6—C7—C14 | −0.4 (5) | C21—C22—C23—C24 | 62.2 (4) |
C5—C6—C7—C14 | −178.2 (3) | O23—C23—C24—C33 | 164.7 (3) |
C6—C7—C8—O8 | −176.7 (3) | C22—C23—C24—C33 | 41.1 (4) |
C14—C7—C8—O8 | 1.0 (5) | O23—C23—C24—C25 | −70.7 (3) |
C6—C7—C8—C9 | 1.0 (5) | C22—C23—C24—C25 | 165.7 (3) |
C14—C7—C8—C9 | 178.7 (3) | C35—O25—C25—C26 | −115.9 (3) |
O1—C1—C9—C10 | −177.7 (3) | C35—O25—C25—C24 | 120.4 (3) |
C2—C1—C9—C10 | −0.1 (5) | C33—C24—C25—O25 | 46.4 (4) |
O1—C1—C9—C8 | 0.3 (5) | C23—C24—C25—O25 | −79.6 (3) |
C2—C1—C9—C8 | 177.9 (3) | C33—C24—C25—C26 | −74.9 (4) |
O8—C8—C9—C10 | 176.6 (3) | C23—C24—C25—C26 | 159.1 (3) |
C7—C8—C9—C10 | −1.1 (5) | O25—C25—C26—C27 | 34.1 (4) |
O8—C8—C9—C1 | −1.4 (5) | C24—C25—C26—C27 | 153.8 (3) |
C7—C8—C9—C1 | −179.1 (3) | O25—C25—C26—C34 | 158.7 (3) |
O4—C4—C10—C9 | −179.2 (3) | C24—C25—C26—C34 | −81.6 (4) |
C3—C4—C10—C9 | 1.8 (5) | C37—O27—C27—C28 | 79.4 (4) |
O4—C4—C10—C5 | 3.8 (5) | C37—O27—C27—C26 | −156.6 (3) |
C3—C4—C10—C5 | −175.3 (3) | C25—C26—C27—O27 | 65.4 (4) |
C1—C9—C10—C4 | 1.3 (5) | C34—C26—C27—O27 | −57.5 (4) |
C8—C9—C10—C4 | −176.6 (3) | C25—C26—C27—C28 | −171.3 (3) |
C1—C9—C10—C5 | 178.6 (3) | C34—C26—C27—C28 | 65.7 (4) |
C8—C9—C10—C5 | 0.6 (5) | O27—C27—C28—C29 | −119.1 (3) |
C11—C5—C10—C4 | 4.1 (6) | C26—C27—C28—C29 | 118.4 (3) |
C6—C5—C10—C4 | 177.0 (3) | C27—C28—C29—O12 | −174.7 (3) |
C11—C5—C10—C9 | −173.1 (3) | C12—O12—C29—C28 | 62.1 (4) |
C6—C5—C10—C9 | −0.2 (5) | C25—O25—C35—O35 | −3.3 (6) |
C6—C5—C11—O11 | −173.9 (3) | C25—O25—C35—C36 | 176.6 (3) |
C10—C5—C11—O11 | −0.3 (6) | N39—N38—C38—C3 | 171.5 (3) |
C6—C5—C11—C12 | 3.8 (4) | C4—C3—C38—N38 | −160.6 (3) |
C10—C5—C11—C12 | 177.4 (3) | C2—C3—C38—N38 | 17.2 (5) |
C29—O12—C12—O6 | −76.9 (3) | N38—N39—C39—C40 | −171.6 (3) |
C29—O12—C12—C13 | 163.4 (3) | C42—N39—C39—C40 | 59.1 (4) |
C29—O12—C12—C11 | 37.9 (3) | C43—N40—C40—C39 | −179.0 (4) |
C6—O6—C12—O12 | 120.6 (3) | C41—N40—C40—C39 | 56.9 (4) |
C6—O6—C12—C13 | −121.3 (3) | N39—C39—C40—N40 | −57.5 (4) |
C6—O6—C12—C11 | 1.2 (3) | C43—N40—C41—C42 | 179.4 (3) |
O11—C11—C12—O12 | 56.3 (4) | C40—N40—C41—C42 | −56.8 (4) |
C5—C11—C12—O12 | −121.7 (3) | N38—N39—C42—C41 | 165.9 (3) |
O11—C11—C12—O6 | 174.9 (3) | C39—N39—C42—C41 | −59.6 (4) |
C5—C11—C12—O6 | −3.1 (3) | N40—C41—C42—N39 | 58.0 (4) |
O11—C11—C12—C13 | −65.4 (4) |
D—H···A | D—H | H···A | D···A | D—H···A |
O1—H1O1···O8 | 1.00 | 1.59 | 2.499 (3) | 148 |
O4—H1O4···O11 | 0.84 | 1.71 | 2.542 (3) | 168 |
O21—H21O···O23 | 0.84 | 1.97 | 2.708 (3) | 147 |
N2—H1N2···N38 | 0.88 | 2.24 | 2.706 (4) | 113 |
O23—H23O···O4i | 0.84 | 1.96 | 2.783 (3) | 165 |
N40—H40N···O1Wii | 1.06 | 1.71 | 2.769 (5) | 170 |
O1W—H2W1···O27iii | 0.84 | 2.00 | 2.835 (5) | 178 |
O2W—H2W2···O21 | 0.84 | 1.99 | 2.795 (4) | 162 |
O3W—H1W3···O8iv | 0.84 | 1.95 | 2.784 (4) | 170 |
O3W—H2W3···O11i | 0.84 | 1.85 | 2.680 (3) | 170 |
O5W—H1W5···O15 | 0.84 | 2.00 | 2.841 (5) | 178 |
O4W—H2W4···O1W | 0.84 | 2.06 | 2.812 (6) | 149 |
O1W—H1W1···O2W | 0.84 | 1.96 | 2.703 (5) | 147 |
O2W—H1W2···O3W | 0.84 | 2.06 | 2.854 (5) | 156 |
O4W—H1W4···O3Wiv | 0.84 | 2.08 | 2.915 (6) | 174 |
C31—H31B···O15v | 0.98 | 2.45 | 3.360 (4) | 154 |
C36—H36A···O15i | 0.98 | 2.54 | 3.431 (5) | 152 |
Symmetry codes: (i) −x+1/2, −y+1, z+1/2; (ii) −x+3/2, −y+1, z−1/2; (iii) x+1, y, z; (iv) x+1/2, −y+3/2, −z+1; (v) −x+1, y−1/2, −z+1/2. |
Experimental details
Crystal data | |
Chemical formula | C43H58N4O12·5H2O |
Mr | 913.01 |
Crystal system, space group | Orthorhombic, P212121 |
Temperature (K) | 130 |
a, b, c (Å) | 13.8506 (6), 17.3867 (8), 19.7476 (8) |
V (Å3) | 4755.5 (4) |
Z | 4 |
Radiation type | Cu Kα |
µ (mm−1) | 0.82 |
Crystal size (mm) | 0.25 × 0.25 × 0.1 |
Data collection | |
Diffractometer | Oxford SuperNova diffractometer |
Absorption correction | Multi-scan (CrysAlis PRO; Agilent, 2010) |
Tmin, Tmax | 0.834, 1.000 |
No. of measured, independent and observed [I > 2σ(I)] reflections | 13055, 4805, 4531 |
Rint | 0.029 |
(sin θ/λ)max (Å−1) | 0.602 |
Refinement | |
R[F2 > 2σ(F2)], wR(F2), S | 0.048, 0.135, 1.06 |
No. of reflections | 4805 |
No. of parameters | 597 |
H-atom treatment | H-atom parameters constrained |
Δρmax, Δρmin (e Å−3) | 0.44, −0.49 |
Computer programs: CrysAlis PRO (Agilent, 2010), SIR2004 (Burla et al., 2005), SHELXL97 (Sheldrick, 2008), ORTEP-3 for Windows (Farrugia, 1997) and Mercury (Macrae et al., 2006).
D—H···A | D—H | H···A | D···A | D—H···A |
O1—H1O1···O8 | 1.00 | 1.59 | 2.499 (3) | 148 |
O4—H1O4···O11 | 0.84 | 1.71 | 2.542 (3) | 168 |
O21—H21O···O23 | 0.84 | 1.97 | 2.708 (3) | 147 |
N2—H1N2···N38 | 0.88 | 2.24 | 2.706 (4) | 113 |
O23—H23O···O4i | 0.84 | 1.96 | 2.783 (3) | 165 |
N40—H40N···O1Wii | 1.06 | 1.71 | 2.769 (5) | 170 |
O1W—H2W1···O27iii | 0.84 | 2.00 | 2.835 (5) | 178 |
O2W—H2W2···O21 | 0.84 | 1.99 | 2.795 (4) | 162 |
O3W—H1W3···O8iv | 0.84 | 1.95 | 2.784 (4) | 170 |
O3W—H2W3···O11i | 0.84 | 1.85 | 2.680 (3) | 170 |
O5W—H1W5···O15 | 0.84 | 2.00 | 2.841 (5) | 178 |
O4W—H2W4···O1W | 0.84 | 2.06 | 2.812 (6) | 149 |
O1W—H1W1···O2W | 0.84 | 1.96 | 2.703 (5) | 147 |
O2W—H1W2···O3W | 0.84 | 2.06 | 2.854 (5) | 156 |
O4W—H1W4···O3Wiv | 0.84 | 2.08 | 2.915 (6) | 174 |
C31—H31B···O15v | 0.98 | 2.45 | 3.360 (4) | 154 |
C36—H36A···O15i | 0.98 | 2.54 | 3.431 (5) | 152 |
Symmetry codes: (i) −x+1/2, −y+1, z+1/2; (ii) −x+3/2, −y+1, z−1/2; (iii) x+1, y, z; (iv) x+1/2, −y+3/2, −z+1; (v) −x+1, y−1/2, −z+1/2. |
Rifampicin, an antibiotic from the group of rifamycins, is utilized worldwide as a result of its broad spectrum of antibacterial activity. Owing to its pronounced activity against mycobacteria, this drug is the first-line antibiotic in the treatment of tuberculosis (Floss & Yu, 2005, and references therein). The solid-state physicochemical properties of rifampicin have been the subject of numerous studies. Two polymorphic forms and a variety of solvates have been reported and characterized for this antibiotic (Ferrari & Gallo, 1975; Pelizza et al., 1977; Henwood et al., 2001; Agrawal et al., 2004). The first structural model of the rifampicin molecule was provided by the crystal structure of rifampicin pentahydrate (Gadret et al., 1975). Although the importance of this model for structural studies is hard to overestimate, as it has been employed in modelling the rifampicin molecule in complexes with proteins (Campbell et al., 2001; Chrencik et al., 2005; Baysarowich et al., 2008), severe problems in locating the H atoms obviated any consideration of a zwitterionic form and the molecule was assumed to be neutral. The possibility of intramolecular proton transfer from the phenolic group at C8 to the piperazine N—CH3 unit was considered for the first time by Ferrari & Gallo (1975). Later on, based on IR spectroscopic studies, the zwitterionic form was postulated for some rifampicin solvates, including the pentahydrate form (Pelizza et al., 1977). Nevertheless, intramolecular proton transfer in rifampicin is largely neglected in the literature. In a recent report of two rifampicin solvates with ethylene glycol, due to the rather poor quality of the diffraction data no conclusions were drawn with respect to the zwitterionic versus neutral form of rifampicin in these systems (de Villiers et al., 2011). In our recent paper on rifampicin and its analogues, where both the neutral and zwitterionic forms of rifampicin were reported in its solvates, intramolecular proton transfer from the phenolic group to the piperazine N—CH3 unit was judged to be an important factor in the mechanism of inhibition of DNA-dependent RNA polymerase (RNAP) by this antibiotic (Pyta et al., 2012).
As a contribution to the further understanding of this important drug we have redetermined the crystal structure of rifampicin pentahydrate, (I). The rifampicin molecule (Fig. 1) in the pentahydrate form exists as a zwitterion, with the phenolate group at C8 and the proton attached to the piperazine N—CH3 unit. This proton transfer is not only confirmed by the location of the H atom on piperazine atom N40 through the diffraction data, but also corroborated by the molecular geometry, mainly the C8—O8 bond length of 1.283 (4) Å and piperazine N40—C bond lengths in the range 1.490 (4)–1.498 (4) Å. These geometric parameters are, within experimental error, the same as those reported for the zwitterionic form of rifampicin in its trihydrate dimethanol solvate [1.287 (4) and 1.490 (4)–1.493 (4) Å, respectively; Pyta et al., 2012]. The molecular structure of this anibiotic is stabilized by a system of intramolecular hydrogen bonds between: (i) the hydroxy group at C1 and the phenolate group at C8; (ii) the hydroxy group at C4 and the O atom of the carbonyl group at C11; (iii) the amide N—H group and the hydrazide N38 atom; and (iv) in the ansa chain, the two hydroxy groups at C21 and C23 (Table 1 and Fig. 1). In the zwitterionic form of rifampicin, a short intramolecular hydrogen bond between the hydroxy group at C1 and the amide carbonyl group (O15) is broken, interrupting the collective system of three hydrogen bonds (O—H···O–H···O═C—N—H···N) observed in the neutral form, and thus giving rise to an increased conformational flexibility about the bond (N2—C2) from the amide group to the aromatic ring.
It has been postulated that, in order to be active against bacterial pathogens, rifamycins need a naphthalene ring with quinone or hydroquinone O atoms at C1 and C8 (using the atom numbering from the present analysis) and two hydroxy groups at C21 and C23 on the ansa fragment that have to be in a particular spatial arrangement (Bacchi et al., 1998). In active forms, the O···O distances between pairs of atoms O1/O8 and O21/O23 should be in the range 5.41–9.58 Å. In inactive forms, the dihedral angle between the best plane through the chromophore and the ansa chain should be less than 50° (Bacchi et al., 1998). In rifampicin pentahydrate, (I), the O1···O21, O1···O23, O8···O21 and O8···O23 distances are 5.346 (4), 6.123 (4), 6.870 (4) and 6.759 (4) Å, respectively, and the relevant dihedral angle is 86.7 (1)°. The etheric junction, spanning atoms C12, O12, C29 and C28, is almost perpendicular to the chromophore, with the dihedral angle between their best planes being 86.5 (1)°. In turn, the absence of an intramolecular hydrogen bond between amide atom O15 and the hydroxy group at C1 results in strong twisting of the amide group relative to the chromophore, with a dihedral angle of 55.6 (2)° between their best planes. This rotation, placing the amide carbonyl group on the other side of the naphthalene ring from that occupied by the ansa chain, results in a few short intermolecular contacts for amide atom O15, including an O—H···O hydrogen bond with water molecule O5W and two short C—H···O contacts with methyl groups (Table 1). The piperazine ring, as expected, adopts a chair conformation, with the two substituents at the piperazine N atoms situated equatorially. The overall conformational parameters of the rifampicin molecule in its pentahydrate form in (I) are in good agreement with those found for rifampicin trihydrate dimethanol solvate (Pyta et al., 2012) (Fig. 2). The r.m.s. deviation of the least-squares superposition of rifampicin molecules from these two solvates is 0.133 Å, as calculated using the program COOT (Emsley et al., 2010).
For compound (I) as a whole, rifampicin pentahydrate is isostructural with rifampicin trihydrate dimethanol solvate (Pyta et al., 2012), where water molecules O4W and O5W of the present structure are replaced by two methanol molecules without significantly altering the crystal packing (Fig. 3). In both solvates, the rifampicin molecules are connected directly by a hydrogen bond between hydroxy group O23—H23 of the ansa chain and the O atom of the O4—H4 hydroxy group at (-x + 1/2, -y + 1, z + 1/2) at the naphthalene ring, forming a chain extending along [001]. Also, in the 1,1,1-trichloroethane solvate (Pyta et al., 2012), the neutral rifampicin molecules are assembled into chains via interaction of the same donor group with the more basic piperazine atom N40 as an acceptor. In the pentahydrate form, (I), four water molecules, O1W to O4W, are hydrogen bonded into a helical chain extending along [100], with water molecules O1W and O2W each acting as a single donor and a single acceptor, O3W as a double acceptor and O4W as a double donor of hydrogen bonds within this chain (Figs. 3 and 4). Water molecules O1W, O2W and O3W are additionally hydrogen bonded to rifampicin via five hydrogen bonds, one of them involving the piperazinium N40—H40 group as donor and atom O1W(-x + 3/2, -y + 1, z - 1/2) as acceptor. In turn, water molecule O5W is located in a hydrophobic niche over the naphthalene group lined by methyl groups of rifampicin molecules belonging to two neighbouring [001] chains. Atom O5W is only weakly hydrogen bonded, forming one hydrogen bond to the amide carbonyl group. When this water molecule is replaced by methanol in the trihydrate dimethanol solvate, the position of the O atom remains virtually unchanged and the methyl group is directed to the naphthalene ring system with a methyl H atom at a distance of 2.66 Å from the naphthalene best plane (Fig. 2). Substitution of methanol for water molecule O4W results in the interruption of the helical chain of O—H···O hydrogen bonds (Fig. 4). We are presently checking whether cocrystallization of rifampicin with some other alcohols will result in similar structures.