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The crystal structures of lenalidomide [systematic name: (
RS)-3-(4-amino-1-oxoisoindolin-2-yl)piperidine-2,6-dione], C
13H
13N
3O
3, (I), an antineoplastic drug, and its hemihydrate, C
13H
13N
3O
3·0.5H
2O, (II), have been determined by single-crystal X-ray diffraction analysis. The overall conformation of the molecule defined by the orientation of the two ring portions,
viz. pyridinedione and isoindolinone, is twisted in both structures. The influence of the self-complementary pyridinedione ring is seen in the crystal packing of both structures through its involvement in forming hydrogen-bonded dimers, although alternate dione O atoms are utilized. An extensive series of N-H
O hydrogen bonds link the dimers into two-dimensional supramolecular arrays built up from infinite chains. The water molecule in (II) has a cohesive function, connecting three lenalidomide molecules by hydrogen bonds. The significance of this study lies in the analysis of the interactions in these structures and the aggregations occurring
via hydrogen bonds in the hydrated and dehydrated crystalline forms of the title compound.
Supporting information
CCDC references: 755982; 755983
Lenalidomide (Pharmacology Department, IICT, Hyderabad) (80 mg) was dissolved
in methanol (5 ml) and water (1 ml). After 3 d, crystals of (I) and (II) were
obtained, distinguished by their distinct crystal habits.
All N-bound H atoms of (I) and (II), and the O-bound H atoms of (II), were
located in difference Fourier maps and their positions and isotropic
displacement parameters refined. All other H atoms were located in a
difference density map, positioned geometrically and included as riding atoms,
with C—H = 0.93–0.98 Å and with Uiso(H) = 1.2Ueq(C).
Distance restraints were applied to O1W—H1W and
O1W—H2W of the water molecule of (II), with a set value of
0.89 (1) Å.
For both compounds, data collection: SMART (Bruker, 2001); cell refinement: SAINT (Bruker, 2001); data reduction: SAINT (Bruker, 2001); program(s) used to solve structure: SHELXS97 (Sheldrick, 2008); program(s) used to refine structure: SHELXL97 (Sheldrick, 2008); molecular graphics: DIAMOND (Brandenburg & Putz, 2005); software used to prepare material for publication: SHELXL97 (Sheldrick, 2008).
(I) (
RS)-3-(4-amino-1-oxoisoindolin-2-yl)piperidine-2,6-dione
top
Crystal data top
C13H13N3O3 | Z = 2 |
Mr = 259.26 | F(000) = 272 |
Triclinic, P1 | Dx = 1.442 Mg m−3 |
Hall symbol: -P 1 | Mo Kα radiation, λ = 0.71073 Å |
a = 5.9983 (5) Å | Cell parameters from 5070 reflections |
b = 8.9198 (8) Å | θ = 2.4–28.1° |
c = 11.5785 (10) Å | µ = 0.11 mm−1 |
α = 75.711 (1)° | T = 294 K |
β = 84.660 (1)° | Block, colourless |
γ = 86.038 (1)° | 0.19 × 0.15 × 0.12 mm |
V = 597.04 (9) Å3 | |
Data collection top
Bruker SMART APEX CCD area-detector diffractometer | 1975 reflections with I > 2σ(I) |
Radiation source: fine-focus sealed tube | Rint = 0.016 |
Graphite monochromator | θmax = 25.0°, θmin = 1.8° |
ω scans | h = −7→7 |
5703 measured reflections | k = −10→10 |
2092 independent reflections | l = −13→13 |
Refinement top
Refinement on F2 | Secondary atom site location: difference Fourier map |
Least-squares matrix: full | Hydrogen site location: inferred from neighbouring sites |
R[F2 > 2σ(F2)] = 0.039 | H atoms treated by a mixture of independent and constrained refinement |
wR(F2) = 0.104 | w = 1/[σ2(Fo2) + (0.0565P)2 + 0.1503P] where P = (Fo2 + 2Fc2)/3 |
S = 1.05 | (Δ/σ)max = 0.001 |
2092 reflections | Δρmax = 0.22 e Å−3 |
185 parameters | Δρmin = −0.20 e Å−3 |
0 restraints | Extinction correction: SHELXL97 (Sheldrick, 2008), Fc*=kFc[1+0.001xFc2λ3/sin(2θ)]-1/4 |
Primary atom site location: structure-invariant direct methods | Extinction coefficient: 0.069 (8) |
Crystal data top
C13H13N3O3 | γ = 86.038 (1)° |
Mr = 259.26 | V = 597.04 (9) Å3 |
Triclinic, P1 | Z = 2 |
a = 5.9983 (5) Å | Mo Kα radiation |
b = 8.9198 (8) Å | µ = 0.11 mm−1 |
c = 11.5785 (10) Å | T = 294 K |
α = 75.711 (1)° | 0.19 × 0.15 × 0.12 mm |
β = 84.660 (1)° | |
Data collection top
Bruker SMART APEX CCD area-detector diffractometer | 1975 reflections with I > 2σ(I) |
5703 measured reflections | Rint = 0.016 |
2092 independent reflections | |
Refinement top
R[F2 > 2σ(F2)] = 0.039 | 0 restraints |
wR(F2) = 0.104 | H atoms treated by a mixture of independent and constrained refinement |
S = 1.05 | Δρmax = 0.22 e Å−3 |
2092 reflections | Δρmin = −0.20 e Å−3 |
185 parameters | |
Special details top
Geometry. All e.s.d.'s (except the e.s.d. in the dihedral angle between two l.s. planes)
are estimated using the full covariance matrix. The cell e.s.d.'s are taken
into account individually in the estimation of e.s.d.'s in distances, angles
and torsion angles; correlations between e.s.d.'s in cell parameters are only
used when they are defined by crystal symmetry. An approximate (isotropic)
treatment of cell e.s.d.'s is used for estimating e.s.d.'s involving l.s.
planes. |
Fractional atomic coordinates and isotropic or equivalent isotropic displacement parameters (Å2) top | x | y | z | Uiso*/Ueq | |
C1 | 0.5579 (2) | 0.82780 (15) | 1.11014 (11) | 0.0377 (3) | |
C2 | 0.4347 (2) | 0.70981 (16) | 1.07203 (13) | 0.0416 (3) | |
H2A | 0.2748 | 0.7298 | 1.0866 | 0.050* | |
H2B | 0.4735 | 0.6078 | 1.1206 | 0.050* | |
C3 | 0.4871 (2) | 0.71039 (16) | 0.94097 (12) | 0.0382 (3) | |
H3A | 0.4271 | 0.8059 | 0.8912 | 0.046* | |
H3B | 0.4173 | 0.6246 | 0.9233 | 0.046* | |
C4 | 0.7402 (2) | 0.69600 (14) | 0.91373 (11) | 0.0337 (3) | |
H4 | 0.7952 | 0.5998 | 0.9667 | 0.040* | |
C5 | 0.8484 (2) | 0.82910 (15) | 0.94414 (11) | 0.0339 (3) | |
C6 | 0.7203 (3) | 0.79641 (17) | 0.68838 (12) | 0.0452 (4) | |
H6A | 0.7271 | 0.9028 | 0.6937 | 0.054* | |
H6B | 0.5670 | 0.7762 | 0.6790 | 0.054* | |
C7 | 0.8771 (2) | 0.76305 (17) | 0.58847 (12) | 0.0429 (3) | |
C8 | 0.8739 (3) | 0.82670 (19) | 0.46597 (13) | 0.0545 (4) | |
C9 | 1.0466 (3) | 0.7790 (2) | 0.39317 (14) | 0.0598 (5) | |
H9 | 1.0519 | 0.8212 | 0.3111 | 0.072* | |
C10 | 1.2101 (3) | 0.6711 (2) | 0.43898 (15) | 0.0667 (5) | |
H10 | 1.3236 | 0.6421 | 0.3873 | 0.080* | |
C11 | 1.2098 (3) | 0.6045 (2) | 0.56047 (14) | 0.0579 (4) | |
H11 | 1.3192 | 0.5302 | 0.5915 | 0.069* | |
C12 | 1.0399 (2) | 0.65336 (16) | 0.63332 (12) | 0.0399 (3) | |
C13 | 0.9968 (2) | 0.60616 (15) | 0.76452 (12) | 0.0363 (3) | |
N1 | 0.75510 (19) | 0.87523 (14) | 1.04327 (10) | 0.0380 (3) | |
H1N | 0.825 (3) | 0.947 (2) | 1.0643 (15) | 0.050 (4)* | |
N2 | 0.80718 (18) | 0.68780 (12) | 0.79265 (9) | 0.0363 (3) | |
N3 | 0.7069 (4) | 0.9356 (3) | 0.42101 (15) | 0.0904 (7) | |
H2N | 0.575 (7) | 0.906 (5) | 0.462 (4) | 0.110 (18)* | |
H3N | 0.705 (5) | 0.949 (3) | 0.349 (3) | 0.114 (9)* | |
O1 | 0.49436 (19) | 0.88326 (13) | 1.19327 (9) | 0.0559 (3) | |
O2 | 1.01178 (15) | 0.89120 (12) | 0.88665 (9) | 0.0459 (3) | |
O3 | 1.10446 (18) | 0.51219 (12) | 0.83683 (9) | 0.0520 (3) | |
Atomic displacement parameters (Å2) top | U11 | U22 | U33 | U12 | U13 | U23 |
C1 | 0.0401 (7) | 0.0403 (7) | 0.0303 (7) | 0.0009 (5) | −0.0001 (5) | −0.0056 (5) |
C2 | 0.0359 (7) | 0.0428 (7) | 0.0442 (8) | −0.0051 (6) | 0.0045 (6) | −0.0089 (6) |
C3 | 0.0341 (7) | 0.0402 (7) | 0.0415 (7) | −0.0035 (5) | −0.0041 (5) | −0.0114 (6) |
C4 | 0.0346 (7) | 0.0351 (6) | 0.0306 (6) | 0.0024 (5) | −0.0045 (5) | −0.0068 (5) |
C5 | 0.0289 (6) | 0.0406 (7) | 0.0316 (6) | 0.0020 (5) | −0.0045 (5) | −0.0076 (5) |
C6 | 0.0490 (8) | 0.0515 (8) | 0.0332 (7) | 0.0167 (6) | −0.0093 (6) | −0.0100 (6) |
C7 | 0.0467 (8) | 0.0464 (8) | 0.0346 (7) | 0.0078 (6) | −0.0050 (6) | −0.0099 (6) |
C8 | 0.0656 (10) | 0.0596 (9) | 0.0342 (8) | 0.0171 (8) | −0.0058 (7) | −0.0087 (7) |
C9 | 0.0720 (11) | 0.0665 (10) | 0.0350 (8) | 0.0065 (8) | 0.0050 (7) | −0.0073 (7) |
C10 | 0.0628 (11) | 0.0816 (12) | 0.0478 (9) | 0.0159 (9) | 0.0137 (8) | −0.0130 (8) |
C11 | 0.0503 (9) | 0.0673 (10) | 0.0496 (9) | 0.0181 (8) | 0.0030 (7) | −0.0100 (8) |
C12 | 0.0391 (7) | 0.0422 (7) | 0.0376 (7) | 0.0033 (6) | −0.0028 (6) | −0.0093 (6) |
C13 | 0.0355 (7) | 0.0373 (7) | 0.0367 (7) | 0.0031 (5) | −0.0068 (5) | −0.0098 (5) |
N1 | 0.0380 (6) | 0.0440 (6) | 0.0349 (6) | −0.0065 (5) | −0.0001 (5) | −0.0146 (5) |
N2 | 0.0382 (6) | 0.0406 (6) | 0.0299 (6) | 0.0076 (5) | −0.0066 (4) | −0.0095 (4) |
N3 | 0.1094 (16) | 0.1134 (15) | 0.0343 (8) | 0.0609 (13) | −0.0141 (9) | −0.0062 (9) |
O1 | 0.0616 (7) | 0.0674 (7) | 0.0420 (6) | −0.0103 (5) | 0.0124 (5) | −0.0237 (5) |
O2 | 0.0358 (5) | 0.0585 (6) | 0.0466 (6) | −0.0107 (4) | 0.0050 (4) | −0.0192 (5) |
O3 | 0.0527 (6) | 0.0568 (6) | 0.0415 (6) | 0.0199 (5) | −0.0107 (5) | −0.0062 (5) |
Geometric parameters (Å, º) top
C1—O1 | 1.2072 (16) | C6—H6B | 0.9700 |
C1—N1 | 1.3835 (17) | C7—C12 | 1.3758 (19) |
C1—C2 | 1.4987 (19) | C7—C8 | 1.393 (2) |
C2—C3 | 1.5195 (19) | C8—C9 | 1.385 (2) |
C2—H2A | 0.9700 | C8—N3 | 1.389 (2) |
C2—H2B | 0.9700 | C9—C10 | 1.373 (3) |
C3—C4 | 1.5248 (18) | C9—H9 | 0.9300 |
C3—H3A | 0.9700 | C10—C11 | 1.385 (2) |
C3—H3B | 0.9700 | C10—H10 | 0.9300 |
C4—N2 | 1.4401 (16) | C11—C12 | 1.378 (2) |
C4—C5 | 1.5201 (18) | C11—H11 | 0.9300 |
C4—H4 | 0.9800 | C12—C13 | 1.4758 (18) |
C5—O2 | 1.2172 (16) | C13—O3 | 1.2246 (16) |
C5—N1 | 1.3718 (17) | C13—N2 | 1.3653 (16) |
C6—N2 | 1.4620 (17) | N1—H1N | 0.882 (19) |
C6—C7 | 1.4965 (19) | N3—H2N | 0.91 (4) |
C6—H6A | 0.9700 | N3—H3N | 0.81 (3) |
| | | |
O1—C1—N1 | 119.34 (13) | C12—C7—C8 | 120.95 (13) |
O1—C1—C2 | 124.14 (12) | C12—C7—C6 | 110.07 (12) |
N1—C1—C2 | 116.51 (11) | C8—C7—C6 | 128.97 (13) |
C1—C2—C3 | 113.11 (11) | C9—C8—N3 | 122.43 (15) |
C1—C2—H2A | 109.0 | C9—C8—C7 | 116.84 (14) |
C3—C2—H2A | 109.0 | N3—C8—C7 | 120.72 (15) |
C1—C2—H2B | 109.0 | C10—C9—C8 | 121.72 (15) |
C3—C2—H2B | 109.0 | C10—C9—H9 | 119.1 |
H2A—C2—H2B | 107.8 | C8—C9—H9 | 119.1 |
C2—C3—C4 | 109.58 (11) | C9—C10—C11 | 121.47 (15) |
C2—C3—H3A | 109.8 | C9—C10—H10 | 119.3 |
C4—C3—H3A | 109.8 | C11—C10—H10 | 119.3 |
C2—C3—H3B | 109.8 | C12—C11—C10 | 116.94 (15) |
C4—C3—H3B | 109.8 | C12—C11—H11 | 121.5 |
H3A—C3—H3B | 108.2 | C10—C11—H11 | 121.5 |
N2—C4—C5 | 110.83 (10) | C7—C12—C11 | 122.04 (13) |
N2—C4—C3 | 113.93 (10) | C7—C12—C13 | 108.44 (12) |
C5—C4—C3 | 109.36 (10) | C11—C12—C13 | 129.52 (13) |
N2—C4—H4 | 107.5 | O3—C13—N2 | 125.03 (12) |
C5—C4—H4 | 107.5 | O3—C13—C12 | 128.45 (12) |
C3—C4—H4 | 107.5 | N2—C13—C12 | 106.52 (11) |
O2—C5—N1 | 120.59 (12) | C5—N1—C1 | 127.36 (12) |
O2—C5—C4 | 123.07 (11) | C5—N1—H1N | 116.2 (11) |
N1—C5—C4 | 116.32 (11) | C1—N1—H1N | 116.4 (11) |
N2—C6—C7 | 101.99 (10) | C13—N2—C4 | 121.88 (10) |
N2—C6—H6A | 111.4 | C13—N2—C6 | 112.86 (11) |
C7—C6—H6A | 111.4 | C4—N2—C6 | 123.15 (10) |
N2—C6—H6B | 111.4 | C8—N3—H2N | 109 (3) |
C7—C6—H6B | 111.4 | C8—N3—H3N | 112 (2) |
H6A—C6—H6B | 109.2 | H2N—N3—H3N | 114 (3) |
| | | |
O1—C1—C2—C3 | 154.97 (13) | C6—C7—C12—C13 | 1.18 (17) |
N1—C1—C2—C3 | −24.26 (17) | C10—C11—C12—C7 | 0.1 (3) |
C1—C2—C3—C4 | 52.80 (15) | C10—C11—C12—C13 | −179.14 (16) |
C2—C3—C4—N2 | 177.12 (10) | C7—C12—C13—O3 | −179.46 (14) |
C2—C3—C4—C5 | −58.25 (14) | C11—C12—C13—O3 | −0.2 (3) |
N2—C4—C5—O2 | −18.13 (17) | C7—C12—C13—N2 | 1.04 (15) |
C3—C4—C5—O2 | −144.55 (12) | C11—C12—C13—N2 | −179.68 (16) |
N2—C4—C5—N1 | 163.35 (11) | O2—C5—N1—C1 | 172.80 (12) |
C3—C4—C5—N1 | 36.93 (15) | C4—C5—N1—C1 | −8.63 (19) |
N2—C6—C7—C12 | −2.75 (16) | O1—C1—N1—C5 | −177.66 (12) |
N2—C6—C7—C8 | 177.30 (16) | C2—C1—N1—C5 | 1.6 (2) |
C12—C7—C8—C9 | −2.6 (2) | O3—C13—N2—C4 | 13.6 (2) |
C6—C7—C8—C9 | 177.36 (16) | C12—C13—N2—C4 | −166.90 (11) |
C12—C7—C8—N3 | 178.96 (19) | O3—C13—N2—C6 | 177.52 (14) |
C6—C7—C8—N3 | −1.1 (3) | C12—C13—N2—C6 | −2.96 (15) |
N3—C8—C9—C10 | −179.9 (2) | C5—C4—N2—C13 | 87.61 (14) |
C7—C8—C9—C10 | 1.7 (3) | C3—C4—N2—C13 | −148.55 (12) |
C8—C9—C10—C11 | 0.1 (3) | C5—C4—N2—C6 | −74.66 (15) |
C9—C10—C11—C12 | −1.0 (3) | C3—C4—N2—C6 | 49.18 (17) |
C8—C7—C12—C11 | 1.8 (2) | C7—C6—N2—C13 | 3.53 (15) |
C6—C7—C12—C11 | −178.16 (15) | C7—C6—N2—C4 | 167.23 (12) |
C8—C7—C12—C13 | −178.86 (14) | | |
Hydrogen-bond geometry (Å, º) top
D—H···A | D—H | H···A | D···A | D—H···A |
N1—H1N···O2i | 0.88 (2) | 2.02 (2) | 2.899 (2) | 179 (2) |
N3—H2N···N3ii | 0.91 (4) | 2.62 (4) | 3.260 (4) | 128 (3) |
N3—H3N···O1iii | 0.81 (3) | 2.50 (3) | 3.180 (2) | 142 (3) |
C3—H3A···O2iv | 0.97 | 2.56 | 3.213 (2) | 125 |
C3—H3B···O3iv | 0.97 | 2.58 | 3.461 (2) | 152 |
C4—H4···O3v | 0.98 | 2.36 | 3.205 (2) | 144 |
Symmetry codes: (i) −x+2, −y+2, −z+2; (ii) −x+1, −y+2, −z+1; (iii) x, y, z−1; (iv) x−1, y, z; (v) −x+2, −y+1, −z+2. |
(II) (
RS)-3-(4-amino-1-oxoisoindolin-2-yl)piperidine-2,6-dione hemihydrate
top
Crystal data top
C13H13N3O3·0.5H2O | F(000) = 1128 |
Mr = 268.27 | Dx = 1.413 Mg m−3 |
Monoclinic, P21/c | Mo Kα radiation, λ = 0.71073 Å |
Hall symbol: -P 2ybc | Cell parameters from 7073 reflections |
a = 8.4425 (7) Å | θ = 2.4–27.9° |
b = 22.2874 (19) Å | µ = 0.11 mm−1 |
c = 13.6627 (12) Å | T = 294 K |
β = 101.069 (1)° | Needle, colourless |
V = 2523.0 (4) Å3 | 0.18 × 0.11 × 0.07 mm |
Z = 8 | |
Data collection top
Bruker SMART APEX CCD area-detector diffractometer | 3972 reflections with I > 2σ(I) |
Radiation source: fine-focus sealed tube | Rint = 0.022 |
Graphite monochromator | θmax = 25.0°, θmin = 1.8° |
ω scans | h = −10→10 |
23782 measured reflections | k = −26→26 |
4430 independent reflections | l = −16→16 |
Refinement top
Refinement on F2 | Primary atom site location: structure-invariant direct methods |
Least-squares matrix: full | Secondary atom site location: difference Fourier map |
R[F2 > 2σ(F2)] = 0.036 | Hydrogen site location: inferred from neighbouring sites |
wR(F2) = 0.105 | H atoms treated by a mixture of independent and constrained refinement |
S = 1.02 | w = 1/[σ2(Fo2) + (0.0566P)2 + 0.8341P] where P = (Fo2 + 2Fc2)/3 |
4430 reflections | (Δ/σ)max = 0.001 |
383 parameters | Δρmax = 0.39 e Å−3 |
2 restraints | Δρmin = −0.18 e Å−3 |
Crystal data top
C13H13N3O3·0.5H2O | V = 2523.0 (4) Å3 |
Mr = 268.27 | Z = 8 |
Monoclinic, P21/c | Mo Kα radiation |
a = 8.4425 (7) Å | µ = 0.11 mm−1 |
b = 22.2874 (19) Å | T = 294 K |
c = 13.6627 (12) Å | 0.18 × 0.11 × 0.07 mm |
β = 101.069 (1)° | |
Data collection top
Bruker SMART APEX CCD area-detector diffractometer | 3972 reflections with I > 2σ(I) |
23782 measured reflections | Rint = 0.022 |
4430 independent reflections | |
Refinement top
R[F2 > 2σ(F2)] = 0.036 | 2 restraints |
wR(F2) = 0.105 | H atoms treated by a mixture of independent and constrained refinement |
S = 1.02 | Δρmax = 0.39 e Å−3 |
4430 reflections | Δρmin = −0.18 e Å−3 |
383 parameters | |
Special details top
Geometry. All e.s.d.'s (except the e.s.d. in the dihedral angle between two l.s. planes)
are estimated using the full covariance matrix. The cell e.s.d.'s are taken
into account individually in the estimation of e.s.d.'s in distances, angles
and torsion angles; correlations between e.s.d.'s in cell parameters are only
used when they are defined by crystal symmetry. An approximate (isotropic)
treatment of cell e.s.d.'s is used for estimating e.s.d.'s involving l.s.
planes. |
Fractional atomic coordinates and isotropic or equivalent isotropic displacement parameters (Å2) top | x | y | z | Uiso*/Ueq | |
C1A | −0.09157 (18) | 0.63219 (7) | 0.72003 (11) | 0.0392 (3) | |
C2A | −0.10504 (18) | 0.69363 (7) | 0.67547 (12) | 0.0410 (3) | |
H2A | −0.2090 | 0.7104 | 0.6809 | 0.049* | |
H2B | −0.1017 | 0.6904 | 0.6051 | 0.049* | |
C3A | 0.02640 (17) | 0.73648 (6) | 0.72388 (11) | 0.0361 (3) | |
H3A | 0.0088 | 0.7481 | 0.7894 | 0.043* | |
H3B | 0.0245 | 0.7724 | 0.6836 | 0.043* | |
C4A | 0.18888 (16) | 0.70521 (6) | 0.73302 (10) | 0.0337 (3) | |
H4A | 0.1955 | 0.6908 | 0.6662 | 0.040* | |
C5A | 0.19246 (17) | 0.65009 (6) | 0.79865 (11) | 0.0354 (3) | |
C6A | 0.36338 (17) | 0.77535 (6) | 0.85997 (10) | 0.0352 (3) | |
H6A | 0.3870 | 0.7474 | 0.9153 | 0.042* | |
H6B | 0.2749 | 0.8012 | 0.8690 | 0.042* | |
C7A | 0.50935 (16) | 0.81104 (6) | 0.84967 (10) | 0.0328 (3) | |
C8A | 0.60135 (17) | 0.84984 (6) | 0.91874 (11) | 0.0370 (3) | |
C9A | 0.73399 (19) | 0.87669 (7) | 0.88835 (13) | 0.0467 (4) | |
H9A | 0.7993 | 0.9025 | 0.9320 | 0.056* | |
C10A | 0.77113 (19) | 0.86612 (7) | 0.79564 (14) | 0.0493 (4) | |
H10A | 0.8603 | 0.8850 | 0.7787 | 0.059* | |
C11A | 0.67870 (18) | 0.82803 (7) | 0.72735 (12) | 0.0429 (4) | |
H11A | 0.7029 | 0.8211 | 0.6647 | 0.051* | |
C12A | 0.54805 (16) | 0.80081 (6) | 0.75744 (10) | 0.0335 (3) | |
C13A | 0.42950 (16) | 0.75842 (6) | 0.70268 (10) | 0.0333 (3) | |
N1A | 0.05125 (15) | 0.61700 (6) | 0.78172 (10) | 0.0396 (3) | |
N2A | 0.32699 (14) | 0.74385 (5) | 0.76453 (8) | 0.0349 (3) | |
N3A | 0.55867 (19) | 0.86233 (7) | 1.00857 (11) | 0.0485 (3) | |
O1A | −0.20231 (14) | 0.59650 (5) | 0.70355 (10) | 0.0603 (4) | |
O2A | 0.30804 (13) | 0.63410 (5) | 0.85893 (9) | 0.0495 (3) | |
O3A | 0.41846 (13) | 0.74021 (5) | 0.61636 (8) | 0.0468 (3) | |
H1N | 0.054 (2) | 0.5817 (9) | 0.8129 (13) | 0.047 (5)* | |
H2N | 0.634 (2) | 0.8811 (9) | 1.0539 (15) | 0.058 (5)* | |
H3N | 0.503 (3) | 0.8340 (10) | 1.0356 (16) | 0.073 (7)* | |
C1B | −0.52664 (19) | 0.50584 (7) | 0.65301 (13) | 0.0447 (4) | |
C2B | −0.5386 (2) | 0.51510 (8) | 0.75929 (13) | 0.0520 (4) | |
H2C | −0.6501 | 0.5097 | 0.7659 | 0.062* | |
H2D | −0.5085 | 0.5561 | 0.7778 | 0.062* | |
C3B | −0.43253 (19) | 0.47278 (7) | 0.83158 (12) | 0.0450 (4) | |
H3C | −0.4311 | 0.4853 | 0.8998 | 0.054* | |
H3D | −0.4750 | 0.4323 | 0.8233 | 0.054* | |
C4B | −0.26297 (18) | 0.47447 (6) | 0.80992 (11) | 0.0378 (3) | |
H4B | −0.2267 | 0.5163 | 0.8160 | 0.045* | |
C5B | −0.26602 (19) | 0.45528 (7) | 0.70313 (11) | 0.0421 (4) | |
C6B | −0.18099 (18) | 0.37917 (6) | 0.90983 (11) | 0.0380 (3) | |
H6C | −0.2206 | 0.3534 | 0.8532 | 0.046* | |
H6D | −0.2589 | 0.3798 | 0.9536 | 0.046* | |
C7B | −0.01835 (17) | 0.35940 (6) | 0.96413 (10) | 0.0335 (3) | |
C8B | 0.02691 (17) | 0.30487 (6) | 1.01154 (10) | 0.0352 (3) | |
C9B | 0.18859 (19) | 0.29889 (7) | 1.05720 (11) | 0.0431 (4) | |
H9B | 0.2231 | 0.2633 | 1.0901 | 0.052* | |
C10B | 0.29905 (19) | 0.34443 (8) | 1.05485 (13) | 0.0491 (4) | |
H10B | 0.4058 | 0.3389 | 1.0866 | 0.059* | |
C11B | 0.25411 (19) | 0.39800 (7) | 1.00627 (12) | 0.0452 (4) | |
H11B | 0.3286 | 0.4284 | 1.0037 | 0.054* | |
C12B | 0.09341 (17) | 0.40425 (6) | 0.96164 (10) | 0.0361 (3) | |
C13B | 0.01063 (18) | 0.45503 (6) | 0.90361 (10) | 0.0374 (3) | |
N1B | −0.39466 (16) | 0.47573 (6) | 0.63377 (10) | 0.0445 (3) | |
N2B | −0.14721 (15) | 0.43951 (5) | 0.87799 (9) | 0.0379 (3) | |
N3B | −0.08375 (18) | 0.25977 (6) | 1.01624 (11) | 0.0446 (3) | |
O1B | −0.62886 (15) | 0.52360 (6) | 0.58373 (10) | 0.0632 (4) | |
O2B | −0.16241 (16) | 0.42439 (7) | 0.67838 (9) | 0.0653 (4) | |
O3B | 0.07036 (14) | 0.50219 (5) | 0.88195 (9) | 0.0522 (3) | |
H4N | −0.390 (2) | 0.4706 (9) | 0.5704 (17) | 0.065 (6)* | |
H5N | −0.179 (3) | 0.2621 (9) | 0.9750 (15) | 0.059 (6)* | |
H6N | −0.044 (2) | 0.2225 (10) | 1.0280 (15) | 0.061 (6)* | |
O1W | −0.1268 (2) | 0.11826 (8) | 0.98185 (14) | 0.0869 (5) | |
H1W | −0.128 (4) | 0.1007 (15) | 1.0473 (13) | 0.132* | |
H2W | −0.125 (4) | 0.0815 (9) | 0.943 (2) | 0.132* | |
Atomic displacement parameters (Å2) top | U11 | U22 | U33 | U12 | U13 | U23 |
C1A | 0.0364 (8) | 0.0391 (8) | 0.0393 (8) | −0.0064 (6) | 0.0004 (6) | 0.0028 (6) |
C2A | 0.0350 (8) | 0.0399 (8) | 0.0447 (8) | −0.0028 (6) | −0.0009 (6) | 0.0072 (6) |
C3A | 0.0369 (8) | 0.0335 (7) | 0.0365 (8) | −0.0014 (6) | 0.0031 (6) | 0.0040 (6) |
C4A | 0.0339 (7) | 0.0340 (7) | 0.0325 (7) | −0.0048 (6) | 0.0045 (6) | −0.0033 (6) |
C5A | 0.0338 (7) | 0.0323 (7) | 0.0386 (7) | 0.0003 (6) | 0.0033 (6) | −0.0037 (6) |
C6A | 0.0350 (7) | 0.0371 (7) | 0.0332 (7) | −0.0041 (6) | 0.0061 (6) | −0.0033 (6) |
C7A | 0.0293 (7) | 0.0297 (7) | 0.0376 (7) | 0.0022 (5) | 0.0018 (5) | 0.0035 (5) |
C8A | 0.0332 (7) | 0.0314 (7) | 0.0426 (8) | 0.0022 (6) | −0.0020 (6) | 0.0001 (6) |
C9A | 0.0358 (8) | 0.0372 (8) | 0.0622 (10) | −0.0055 (6) | −0.0031 (7) | −0.0023 (7) |
C10A | 0.0333 (8) | 0.0449 (9) | 0.0703 (11) | −0.0069 (7) | 0.0116 (8) | 0.0057 (8) |
C11A | 0.0360 (8) | 0.0426 (8) | 0.0521 (9) | 0.0002 (6) | 0.0134 (7) | 0.0058 (7) |
C12A | 0.0297 (7) | 0.0311 (7) | 0.0390 (7) | 0.0031 (5) | 0.0048 (6) | 0.0037 (6) |
C13A | 0.0320 (7) | 0.0332 (7) | 0.0349 (7) | 0.0032 (5) | 0.0068 (6) | 0.0017 (6) |
N1A | 0.0388 (7) | 0.0317 (6) | 0.0446 (7) | −0.0048 (5) | −0.0010 (5) | 0.0076 (5) |
N2A | 0.0342 (6) | 0.0374 (6) | 0.0331 (6) | −0.0069 (5) | 0.0069 (5) | −0.0045 (5) |
N3A | 0.0514 (8) | 0.0494 (8) | 0.0407 (7) | −0.0074 (7) | −0.0010 (6) | −0.0076 (6) |
O1A | 0.0480 (7) | 0.0506 (7) | 0.0720 (8) | −0.0189 (6) | −0.0140 (6) | 0.0178 (6) |
O2A | 0.0381 (6) | 0.0422 (6) | 0.0615 (7) | 0.0016 (5) | −0.0072 (5) | 0.0056 (5) |
O3A | 0.0483 (6) | 0.0565 (7) | 0.0374 (6) | −0.0050 (5) | 0.0130 (5) | −0.0084 (5) |
C1B | 0.0404 (8) | 0.0350 (8) | 0.0541 (9) | 0.0020 (6) | −0.0024 (7) | 0.0054 (7) |
C2B | 0.0454 (9) | 0.0465 (9) | 0.0622 (11) | 0.0122 (7) | 0.0057 (8) | −0.0019 (8) |
C3B | 0.0455 (9) | 0.0429 (9) | 0.0456 (9) | 0.0024 (7) | 0.0062 (7) | 0.0001 (7) |
C4B | 0.0398 (8) | 0.0303 (7) | 0.0399 (8) | 0.0005 (6) | −0.0010 (6) | 0.0024 (6) |
C5B | 0.0403 (8) | 0.0423 (8) | 0.0411 (8) | 0.0054 (7) | 0.0012 (6) | 0.0030 (6) |
C6B | 0.0374 (8) | 0.0347 (7) | 0.0396 (8) | −0.0027 (6) | 0.0018 (6) | 0.0050 (6) |
C7B | 0.0366 (7) | 0.0347 (7) | 0.0283 (7) | 0.0007 (6) | 0.0039 (5) | −0.0016 (5) |
C8B | 0.0410 (8) | 0.0359 (7) | 0.0288 (7) | 0.0027 (6) | 0.0071 (6) | −0.0006 (6) |
C9B | 0.0433 (9) | 0.0424 (8) | 0.0412 (8) | 0.0098 (7) | 0.0022 (7) | 0.0024 (6) |
C10B | 0.0368 (8) | 0.0538 (10) | 0.0519 (10) | 0.0066 (7) | −0.0033 (7) | −0.0046 (8) |
C11B | 0.0373 (8) | 0.0446 (9) | 0.0510 (9) | −0.0057 (7) | 0.0020 (7) | −0.0068 (7) |
C12B | 0.0394 (8) | 0.0345 (7) | 0.0330 (7) | −0.0006 (6) | 0.0036 (6) | −0.0047 (6) |
C13B | 0.0416 (8) | 0.0330 (7) | 0.0355 (7) | −0.0032 (6) | 0.0023 (6) | −0.0022 (6) |
N1B | 0.0441 (8) | 0.0478 (8) | 0.0376 (7) | 0.0047 (6) | −0.0022 (6) | 0.0007 (6) |
N2B | 0.0406 (7) | 0.0325 (6) | 0.0368 (6) | −0.0017 (5) | −0.0020 (5) | 0.0047 (5) |
N3B | 0.0481 (8) | 0.0393 (8) | 0.0454 (8) | −0.0020 (6) | 0.0063 (6) | 0.0098 (6) |
O1B | 0.0511 (7) | 0.0688 (8) | 0.0628 (8) | 0.0157 (6) | −0.0062 (6) | 0.0155 (6) |
O2B | 0.0588 (8) | 0.0871 (10) | 0.0480 (7) | 0.0303 (7) | 0.0053 (6) | −0.0034 (6) |
O3B | 0.0517 (7) | 0.0356 (6) | 0.0653 (8) | −0.0096 (5) | 0.0011 (6) | 0.0077 (5) |
O1W | 0.0873 (11) | 0.0819 (11) | 0.0844 (12) | −0.0316 (9) | −0.0013 (9) | 0.0246 (9) |
Geometric parameters (Å, º) top
C1A—O1A | 1.2152 (18) | C1B—N1B | 1.369 (2) |
C1A—N1A | 1.3743 (19) | C1B—C2B | 1.489 (2) |
C1A—C2A | 1.494 (2) | C2B—C3B | 1.526 (2) |
C2A—C3A | 1.516 (2) | C2B—H2C | 0.9700 |
C2A—H2A | 0.9700 | C2B—H2D | 0.9700 |
C2A—H2B | 0.9700 | C3B—C4B | 1.517 (2) |
C3A—C4A | 1.522 (2) | C3B—H3C | 0.9700 |
C3A—H3A | 0.9700 | C3B—H3D | 0.9700 |
C3A—H3B | 0.9700 | C4B—N2B | 1.4416 (18) |
C4A—N2A | 1.4470 (17) | C4B—C5B | 1.516 (2) |
C4A—C5A | 1.518 (2) | C4B—H4B | 0.9800 |
C4A—H4A | 0.9800 | C5B—O2B | 1.2112 (19) |
C5A—O2A | 1.2036 (18) | C5B—N1B | 1.3748 (19) |
C5A—N1A | 1.3830 (19) | C6B—N2B | 1.4582 (18) |
C6A—N2A | 1.4606 (18) | C6B—C7B | 1.496 (2) |
C6A—C7A | 1.4960 (19) | C6B—H6C | 0.9700 |
C6A—H6A | 0.9700 | C6B—H6D | 0.9700 |
C6A—H6B | 0.9700 | C7B—C12B | 1.379 (2) |
C7A—C12A | 1.380 (2) | C7B—C8B | 1.396 (2) |
C7A—C8A | 1.400 (2) | C8B—N3B | 1.382 (2) |
C8A—N3A | 1.372 (2) | C8B—C9B | 1.394 (2) |
C8A—C9A | 1.401 (2) | C9B—C10B | 1.383 (2) |
C9A—C10A | 1.382 (3) | C9B—H9B | 0.9300 |
C9A—H9A | 0.9300 | C10B—C11B | 1.383 (2) |
C10A—C11A | 1.386 (2) | C10B—H10B | 0.9300 |
C10A—H10A | 0.9300 | C11B—C12B | 1.384 (2) |
C11A—C12A | 1.389 (2) | C11B—H11B | 0.9300 |
C11A—H11A | 0.9300 | C12B—C13B | 1.478 (2) |
C12A—C13A | 1.472 (2) | C13B—O3B | 1.2263 (18) |
C13A—O3A | 1.2337 (17) | C13B—N2B | 1.3560 (19) |
C13A—N2A | 1.3599 (18) | N1B—H4N | 0.88 (2) |
N1A—H1N | 0.893 (19) | N3B—H5N | 0.89 (2) |
N3A—H2N | 0.90 (2) | N3B—H6N | 0.90 (2) |
N3A—H3N | 0.91 (2) | O1W—H1W | 0.978 (10) |
C1B—O1B | 1.2181 (19) | O1W—H2W | 0.977 (10) |
| | | |
O1A—C1A—N1A | 120.37 (14) | O1B—C1B—C2B | 122.83 (16) |
O1A—C1A—C2A | 121.93 (14) | N1B—C1B—C2B | 117.74 (13) |
N1A—C1A—C2A | 117.70 (13) | C1B—C2B—C3B | 113.84 (13) |
C1A—C2A—C3A | 114.04 (12) | C1B—C2B—H2C | 108.8 |
C1A—C2A—H2A | 108.7 | C3B—C2B—H2C | 108.8 |
C3A—C2A—H2A | 108.7 | C1B—C2B—H2D | 108.8 |
C1A—C2A—H2B | 108.7 | C3B—C2B—H2D | 108.8 |
C3A—C2A—H2B | 108.7 | H2C—C2B—H2D | 107.7 |
H2A—C2A—H2B | 107.6 | C4B—C3B—C2B | 108.49 (13) |
C2A—C3A—C4A | 108.67 (12) | C4B—C3B—H3C | 110.0 |
C2A—C3A—H3A | 110.0 | C2B—C3B—H3C | 110.0 |
C4A—C3A—H3A | 110.0 | C4B—C3B—H3D | 110.0 |
C2A—C3A—H3B | 110.0 | C2B—C3B—H3D | 110.0 |
C4A—C3A—H3B | 110.0 | H3C—C3B—H3D | 108.4 |
H3A—C3A—H3B | 108.3 | N2B—C4B—C5B | 110.64 (12) |
N2A—C4A—C5A | 112.48 (11) | N2B—C4B—C3B | 114.15 (13) |
N2A—C4A—C3A | 114.46 (12) | C5B—C4B—C3B | 109.78 (12) |
C5A—C4A—C3A | 109.54 (11) | N2B—C4B—H4B | 107.3 |
N2A—C4A—H4A | 106.6 | C5B—C4B—H4B | 107.3 |
C5A—C4A—H4A | 106.6 | C3B—C4B—H4B | 107.3 |
C3A—C4A—H4A | 106.6 | O2B—C5B—N1B | 120.88 (15) |
O2A—C5A—N1A | 121.07 (14) | O2B—C5B—C4B | 123.33 (14) |
O2A—C5A—C4A | 124.43 (13) | N1B—C5B—C4B | 115.79 (13) |
N1A—C5A—C4A | 114.45 (12) | N2B—C6B—C7B | 101.89 (11) |
N2A—C6A—C7A | 101.97 (11) | N2B—C6B—H6C | 111.4 |
N2A—C6A—H6A | 111.4 | C7B—C6B—H6C | 111.4 |
C7A—C6A—H6A | 111.4 | N2B—C6B—H6D | 111.4 |
N2A—C6A—H6B | 111.4 | C7B—C6B—H6D | 111.4 |
C7A—C6A—H6B | 111.4 | H6C—C6B—H6D | 109.3 |
H6A—C6A—H6B | 109.2 | C12B—C7B—C8B | 120.99 (13) |
C12A—C7A—C8A | 121.30 (13) | C12B—C7B—C6B | 109.96 (12) |
C12A—C7A—C6A | 109.98 (12) | C8B—C7B—C6B | 129.05 (13) |
C8A—C7A—C6A | 128.72 (13) | N3B—C8B—C9B | 121.48 (14) |
N3A—C8A—C7A | 121.41 (14) | N3B—C8B—C7B | 121.85 (14) |
N3A—C8A—C9A | 122.71 (14) | C9B—C8B—C7B | 116.63 (14) |
C7A—C8A—C9A | 115.83 (14) | C10B—C9B—C8B | 121.76 (14) |
C10A—C9A—C8A | 122.24 (14) | C10B—C9B—H9B | 119.1 |
C10A—C9A—H9A | 118.9 | C8B—C9B—H9B | 119.1 |
C8A—C9A—H9A | 118.9 | C9B—C10B—C11B | 121.37 (15) |
C9A—C10A—C11A | 121.60 (15) | C9B—C10B—H10B | 119.3 |
C9A—C10A—H10A | 119.2 | C11B—C10B—H10B | 119.3 |
C11A—C10A—H10A | 119.2 | C10B—C11B—C12B | 116.99 (15) |
C10A—C11A—C12A | 116.42 (15) | C10B—C11B—H11B | 121.5 |
C10A—C11A—H11A | 121.8 | C12B—C11B—H11B | 121.5 |
C12A—C11A—H11A | 121.8 | C7B—C12B—C11B | 122.25 (14) |
C7A—C12A—C11A | 122.61 (14) | C7B—C12B—C13B | 108.22 (12) |
C7A—C12A—C13A | 108.25 (12) | C11B—C12B—C13B | 129.51 (14) |
C11A—C12A—C13A | 129.14 (14) | O3B—C13B—N2B | 125.85 (14) |
O3A—C13A—N2A | 125.12 (13) | O3B—C13B—C12B | 127.71 (14) |
O3A—C13A—C12A | 128.04 (13) | N2B—C13B—C12B | 106.44 (12) |
N2A—C13A—C12A | 106.80 (12) | C1B—N1B—C5B | 126.48 (15) |
C1A—N1A—C5A | 126.72 (13) | C1B—N1B—H4N | 116.2 (13) |
C1A—N1A—H1N | 116.6 (11) | C5B—N1B—H4N | 117.3 (14) |
C5A—N1A—H1N | 116.6 (11) | C13B—N2B—C4B | 122.64 (12) |
C13A—N2A—C4A | 121.99 (12) | C13B—N2B—C6B | 113.40 (12) |
C13A—N2A—C6A | 112.96 (11) | C4B—N2B—C6B | 122.94 (12) |
C4A—N2A—C6A | 124.82 (11) | C8B—N3B—H5N | 117.7 (13) |
C8A—N3A—H2N | 115.9 (13) | C8B—N3B—H6N | 116.5 (13) |
C8A—N3A—H3N | 118.0 (14) | H5N—N3B—H6N | 115.5 (19) |
H2N—N3A—H3N | 113.3 (18) | H1W—O1W—H2W | 99 (3) |
O1B—C1B—N1B | 119.44 (16) | | |
| | | |
O1A—C1A—C2A—C3A | 165.46 (15) | O1B—C1B—C2B—C3B | 160.63 (16) |
N1A—C1A—C2A—C3A | −14.1 (2) | N1B—C1B—C2B—C3B | −19.0 (2) |
C1A—C2A—C3A—C4A | 47.88 (17) | C1B—C2B—C3B—C4B | 49.94 (19) |
C2A—C3A—C4A—N2A | 171.35 (11) | C2B—C3B—C4B—N2B | 175.80 (12) |
C2A—C3A—C4A—C5A | −61.27 (15) | C2B—C3B—C4B—C5B | −59.32 (16) |
N2A—C4A—C5A—O2A | −12.3 (2) | N2B—C4B—C5B—O2B | −14.4 (2) |
C3A—C4A—C5A—O2A | −140.76 (15) | C3B—C4B—C5B—O2B | −141.31 (17) |
N2A—C4A—C5A—N1A | 169.99 (12) | N2B—C4B—C5B—N1B | 166.26 (13) |
C3A—C4A—C5A—N1A | 41.51 (16) | C3B—C4B—C5B—N1B | 39.39 (18) |
N2A—C6A—C7A—C12A | 0.70 (15) | N2B—C6B—C7B—C12B | −0.39 (15) |
N2A—C6A—C7A—C8A | −179.01 (14) | N2B—C6B—C7B—C8B | −179.66 (14) |
C12A—C7A—C8A—N3A | 176.82 (14) | C12B—C7B—C8B—N3B | 178.93 (13) |
C6A—C7A—C8A—N3A | −3.5 (2) | C6B—C7B—C8B—N3B | −1.9 (2) |
C12A—C7A—C8A—C9A | −0.5 (2) | C12B—C7B—C8B—C9B | 1.2 (2) |
C6A—C7A—C8A—C9A | 179.19 (14) | C6B—C7B—C8B—C9B | −179.61 (14) |
N3A—C8A—C9A—C10A | −176.64 (15) | N3B—C8B—C9B—C10B | −178.38 (15) |
C7A—C8A—C9A—C10A | 0.6 (2) | C7B—C8B—C9B—C10B | −0.6 (2) |
C8A—C9A—C10A—C11A | −0.1 (3) | C8B—C9B—C10B—C11B | −0.5 (3) |
C9A—C10A—C11A—C12A | −0.5 (2) | C9B—C10B—C11B—C12B | 1.0 (2) |
C8A—C7A—C12A—C11A | −0.2 (2) | C8B—C7B—C12B—C11B | −0.7 (2) |
C6A—C7A—C12A—C11A | −179.89 (13) | C6B—C7B—C12B—C11B | 179.98 (14) |
C8A—C7A—C12A—C13A | −179.92 (12) | C8B—C7B—C12B—C13B | 178.01 (12) |
C6A—C7A—C12A—C13A | 0.34 (15) | C6B—C7B—C12B—C13B | −1.33 (16) |
C10A—C11A—C12A—C7A | 0.7 (2) | C10B—C11B—C12B—C7B | −0.4 (2) |
C10A—C11A—C12A—C13A | −179.62 (14) | C10B—C11B—C12B—C13B | −178.82 (15) |
C7A—C12A—C13A—O3A | 176.48 (14) | C7B—C12B—C13B—O3B | −177.26 (15) |
C11A—C12A—C13A—O3A | −3.3 (2) | C11B—C12B—C13B—O3B | 1.3 (3) |
C7A—C12A—C13A—N2A | −1.34 (15) | C7B—C12B—C13B—N2B | 2.66 (16) |
C11A—C12A—C13A—N2A | 178.91 (14) | C11B—C12B—C13B—N2B | −178.78 (15) |
O1A—C1A—N1A—C5A | 172.44 (15) | O1B—C1B—N1B—C5B | 176.92 (16) |
C2A—C1A—N1A—C5A | −8.0 (2) | C2B—C1B—N1B—C5B | −3.4 (2) |
O2A—C5A—N1A—C1A | 175.59 (15) | O2B—C5B—N1B—C1B | 173.28 (16) |
C4A—C5A—N1A—C1A | −6.6 (2) | C4B—C5B—N1B—C1B | −7.4 (2) |
O3A—C13A—N2A—C4A | −1.3 (2) | O3B—C13B—N2B—C4B | 8.1 (2) |
C12A—C13A—N2A—C4A | 176.64 (12) | C12B—C13B—N2B—C4B | −171.79 (12) |
O3A—C13A—N2A—C6A | −176.03 (14) | O3B—C13B—N2B—C6B | 176.88 (15) |
C12A—C13A—N2A—C6A | 1.87 (16) | C12B—C13B—N2B—C6B | −3.05 (16) |
C5A—C4A—N2A—C13A | 121.97 (14) | C5B—C4B—N2B—C13B | 87.42 (16) |
C3A—C4A—N2A—C13A | −112.17 (15) | C3B—C4B—N2B—C13B | −148.16 (14) |
C5A—C4A—N2A—C6A | −63.90 (17) | C5B—C4B—N2B—C6B | −80.25 (17) |
C3A—C4A—N2A—C6A | 61.96 (17) | C3B—C4B—N2B—C6B | 44.17 (19) |
C7A—C6A—N2A—C13A | −1.62 (15) | C7B—C6B—N2B—C13B | 2.20 (16) |
C7A—C6A—N2A—C4A | −176.21 (12) | C7B—C6B—N2B—C4B | 170.90 (13) |
Hydrogen-bond geometry (Å, º) top
D—H···A | D—H | H···A | D···A | D—H···A |
N3A—H2N···O1Ai | 0.90 (2) | 2.29 (2) | 3.1539 (18) | 160 (2) |
N3A—H3N···O3Aii | 0.91 (2) | 2.18 (2) | 3.076 (2) | 168 (2) |
N1A—H1N···O3B | 0.893 (19) | 2.00 (2) | 2.8921 (17) | 177 (2) |
N3B—H5N···O3Aiii | 0.89 (2) | 2.22 (2) | 3.0808 (19) | 164 (2) |
N3B—H6N···O1W | 0.90 (2) | 2.47 (2) | 3.199 (2) | 138 (2) |
N1B—H4N···O1Biv | 0.88 (2) | 2.15 (2) | 3.016 (2) | 169 (2) |
O1W—H1W···O2Bv | 0.98 (1) | 1.95 (1) | 2.918 (2) | 170 (3) |
O1W—H2W···O1Bvi | 0.98 (1) | 2.41 (3) | 2.966 (2) | 116 (2) |
C3A—H3A···N3Bvii | 0.97 | 2.61 | 3.491 (2) | 150 |
C4A—H4A···O1Wviii | 0.98 | 2.57 | 3.471 (2) | 154 |
C4B—H4B···O1A | 0.98 | 2.39 | 3.171 (2) | 136 |
C10B—H10B···O2Aix | 0.93 | 2.46 | 3.335 (2) | 156 |
Symmetry codes: (i) x+1, −y+3/2, z+1/2; (ii) x, −y+3/2, z+1/2; (iii) −x, y−1/2, −z+3/2; (iv) −x−1, −y+1, −z+1; (v) x, −y+1/2, z+1/2; (vi) −x−1, y−1/2, −z+3/2; (vii) −x, −y+1, −z+2; (viii) −x, y+1/2, −z+3/2; (ix) −x+1, −y+1, −z+2. |
Experimental details
| (I) | (II) |
Crystal data |
Chemical formula | C13H13N3O3 | C13H13N3O3·0.5H2O |
Mr | 259.26 | 268.27 |
Crystal system, space group | Triclinic, P1 | Monoclinic, P21/c |
Temperature (K) | 294 | 294 |
a, b, c (Å) | 5.9983 (5), 8.9198 (8), 11.5785 (10) | 8.4425 (7), 22.2874 (19), 13.6627 (12) |
α, β, γ (°) | 75.711 (1), 84.660 (1), 86.038 (1) | 90, 101.069 (1), 90 |
V (Å3) | 597.04 (9) | 2523.0 (4) |
Z | 2 | 8 |
Radiation type | Mo Kα | Mo Kα |
µ (mm−1) | 0.11 | 0.11 |
Crystal size (mm) | 0.19 × 0.15 × 0.12 | 0.18 × 0.11 × 0.07 |
|
Data collection |
Diffractometer | Bruker SMART APEX CCD area-detector diffractometer | Bruker SMART APEX CCD area-detector diffractometer |
Absorption correction | – | – |
No. of measured, independent and observed [I > 2σ(I)] reflections | 5703, 2092, 1975 | 23782, 4430, 3972 |
Rint | 0.016 | 0.022 |
(sin θ/λ)max (Å−1) | 0.595 | 0.595 |
|
Refinement |
R[F2 > 2σ(F2)], wR(F2), S | 0.039, 0.104, 1.05 | 0.036, 0.105, 1.02 |
No. of reflections | 2092 | 4430 |
No. of parameters | 185 | 383 |
No. of restraints | 0 | 2 |
H-atom treatment | H atoms treated by a mixture of independent and constrained refinement | H atoms treated by a mixture of independent and constrained refinement |
Δρmax, Δρmin (e Å−3) | 0.22, −0.20 | 0.39, −0.18 |
Hydrogen-bond geometry (Å, º) for (I) top
D—H···A | D—H | H···A | D···A | D—H···A |
N1—H1N···O2i | 0.88 (2) | 2.02 (2) | 2.899 (2) | 179 (2) |
N3—H2N···N3ii | 0.91 (4) | 2.62 (4) | 3.260 (4) | 128 (3) |
N3—H3N···O1iii | 0.81 (3) | 2.50 (3) | 3.180 (2) | 142 (3) |
C3—H3A···O2iv | 0.97 | 2.56 | 3.213 (2) | 125 |
C3—H3B···O3iv | 0.97 | 2.58 | 3.461 (2) | 152 |
C4—H4···O3v | 0.98 | 2.36 | 3.205 (2) | 144 |
Symmetry codes: (i) −x+2, −y+2, −z+2; (ii) −x+1, −y+2, −z+1; (iii) x, y, z−1; (iv) x−1, y, z; (v) −x+2, −y+1, −z+2. |
Hydrogen-bond geometry (Å, º) for (II) top
D—H···A | D—H | H···A | D···A | D—H···A |
N3A—H2N···O1Ai | 0.90 (2) | 2.29 (2) | 3.1539 (18) | 160 (2) |
N3A—H3N···O3Aii | 0.91 (2) | 2.18 (2) | 3.076 (2) | 168 (2) |
N1A—H1N···O3B | 0.893 (19) | 2.00 (2) | 2.8921 (17) | 177 (2) |
N3B—H5N···O3Aiii | 0.89 (2) | 2.22 (2) | 3.0808 (19) | 164 (2) |
N3B—H6N···O1W | 0.90 (2) | 2.47 (2) | 3.199 (2) | 138 (2) |
N1B—H4N···O1Biv | 0.88 (2) | 2.15 (2) | 3.016 (2) | 169 (2) |
O1W—H1W···O2Bv | 0.978 (10) | 1.951 (12) | 2.918 (2) | 170 (3) |
O1W—H2W···O1Bvi | 0.977 (10) | 2.41 (3) | 2.966 (2) | 116 (2) |
C3A—H3A···N3Bvii | 0.97 | 2.61 | 3.491 (2) | 150 |
C4A—H4A···O1Wviii | 0.98 | 2.57 | 3.471 (2) | 154 |
C4B—H4B···O1A | 0.98 | 2.39 | 3.171 (2) | 136 |
C10B—H10B···O2Aix | 0.93 | 2.46 | 3.335 (2) | 156 |
Symmetry codes: (i) x+1, −y+3/2, z+1/2; (ii) x, −y+3/2, z+1/2; (iii) −x, y−1/2, −z+3/2; (iv) −x−1, −y+1, −z+1; (v) x, −y+1/2, z+1/2; (vi) −x−1, y−1/2, −z+3/2; (vii) −x, −y+1, −z+2; (viii) −x, y+1/2, −z+3/2; (ix) −x+1, −y+1, −z+2. |
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Lenalidomide, initially known as CC-5013 and marketed as Revlimid by Celgene Europe Ltd., is one of a number of novel compounds based on the molecular structure of thalidomide that were developed with a view to improving the immunomodulatory effect of the parent compound, whilst also providing a better safety profile (Bartlett et al., 2004; Richardson et al., 2002). It is a synthetic derivative of glutamic acid and is structurally close to thalidomide, in that it has an identical backbone but has been subject to removal of an O atom from the phthalyl ring and addition of an amine group. Although it is chiral and possesses an asymmetric C atom, it has been developed as a racemic mixture since it undergoes racemization under physiological conditions. In a phase III clinical study, Weber et al. (2007) found that treatment with lenalidomide plus dexamethasone in patients with relapsed or refractory multiple myeloma was superior to the old treatment of multiple myeloma consisting of high-dose dexamethasone alone. On June 29 2006, lenalidomide received US Food and Drug Administration (FDA) clearance for use in combination with dexamethasone in patients with multiple myeloma who have received at least one prior therapy.
In the course of our investigations on the structural characterization of pharmaceutical compounds (Ravikumar et al., 2008a,b), the title compound was obtained in two crystal types, as blocks and a small number of fine needles. The data collected suggest the structure of lenalidomide, (I) (triclinic, P1), in the case of the blocks, whereas for the needles, the larger asymmetric unit indicates the presence of two molecules (monoclinic, P21/c, Z = 8) and proved to contain a water molecule as well, considered as lenalidomide hemihydrate, (II). The crystal structures of (I) and (II) are presented here.
Views of the molecules of (I) and (II), showing the atom labelling, are presented in Figs. 1 and 2, respectively. The significant difference between the two molecules of (II) is seen in the central C5—C4—N2—C13 torsion angle, defining the twist between the pyridinedione ring and the aminoisoindolinone portion; this angle is 121.9 (1)° in molecule A and 87.4 (2)° in molecule B. This is the only available point for conformation flexibility in the molecule. The corresponding angle in (I) is 87.6 (1)°. This twist is further illustrated by the non-bonding distance between the carbonyl O atoms of the two rings: O2···O3 = 3.566 (2) Å in (I), 4.317 (2) Å in molecule A of (II) and 3.536 (2) Å in molecule B of (II). An overlay of the molecules of (I) and (II) with thalidomide polymorphs [α form (Allen & Trotter, 1971) and β form (Reepmeyer et al., 1994)], superimposing the planar isoindolinone systems, reveals the conformational flexibility (Fig. 3). The conformation of the pyridinedione ring in both structures has a distorted half-chair conformation in which atoms C3 and C4 lie on opposite sides of the plane defined by atoms C1/C2/N1/C5. The half-chair conformation is distorted towards a C3 envelope.
In both structures (I) and (II), the main interest centres on the crystal packing features, where hydrogen bonds (Tables 1 and 2) play a major part in controlling the supramolecular assembly of the molecules.
In the crystal packing (Fig. 4) of (I), the self-complementary pyridinedione ring forms centrosymmetric dimers (N1—H1N···O2), the hydrogen-bonding motif of which has graph set R22(8) (Etter, 1990; Etter et al., 1990; Bernstein et al., 1995). Amino atom N3 donates one of its H atoms to dione atom O1 at (x, y, z - 1), linking the dimers to generate a cyclic centrosymmetric tetramer of graph set R44(26). These self-complementary tetramers form infinite chains parallel to the c axis, for which the complete graph set can be written as C11(11)[R22(8)R44(26)]. In addition, the molecules are further linked into an additional centrosymmetric dimeric aggregate by N—H···N hydrogen bonds along the a axis, wherein amino atom N3 acts as both donor and acceptor [N3—H2···N2(-x + 1, -y + 2, -z + 1)] to generate a motif of graph set R22(4) propagating along the b axis. Carbonyl atom O3 of the indolinone ring is not involved in any conventional hydrogen bonds, but participates in an acceptable C—H···O interaction (Table 1).
In the hemihydrate, (II), the crystal structure is built up from two lenalidomide molecules, A and B, in the asymmetric unit, with solvent water molecules, via hydrogen-bonding interactions. The self-complementary pyridinedione ring dimer found in (I) forms only between B molecules through N1B—H4N···O1B hydrogen bonds (Fig. 5). Molecule A links to molecule B through pyridine atom N1A to carbonyl atom O3B. It is interesting to note that whilst the dimer formation in (I) is through dione atom O1, it is through atom O2 in (II), thus making use of the alternate presence of dione atoms O1 and O2. The amine atom N3 of both molecules connects neighbouring lenalidomide molecules via N—H···O hydrogen bonds and generates tetramers based on the R44(29) synthon along the c axis.
Two adjacent tetramers thus generate a centrosymmetic hexamer along the a axis, based on the R64(36) synthon, accommodating the N—H···O dimers in its cavity. Symmetry-related N—H···O hydrogen bonds generate an additional centrosymmetic hexamer of R66(42) motif along the b axis, which links the earlier formed R64(36) hexamers, resulting in a continuous linkage of molecules along the c and b axes (Fig. 6).
The cohesive role of water is embodied by its interaction as an acceptor of a hydrogen bond donated by amine atom N3B, and as a donor to the two different carbonyl atoms O1B and O2B of the pyridinedione ring, generating a graph-set motif of R23(8) (Fig. 7). It is interesting to note that the water molecule interacts only with molecule B. Carbonyl atom O2A of the pyridinedione ring is not involved in any conventional hydrogen bonds, but participates in an acceptable C—H···O interaction (Table 2).
To sum up, the crystal structure analysis of (I) and (II) illustrates the arrangements of supramolecular aggregates formed by hydrogen-bonding interactions in the hydrated and dehydrated forms of lenalidomide. The observed crystal packing features, with and without the interposition of water molecules, may provide some insight to understand ways of improving the tablet-formation ability and densification properties of drug substances.