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IYCr crystallization series
SCaMC is an ATP-Mg/Pi carrier protein located at the mitochondrial inner membrane. SCaMC has an unusual N-terminal Ca2+-binding domain (NTD) in addition to its characteristic six-helix transmembrane bundle. The NTD of human SCaMC1 (residues 1-193) was expressed and purified in order to study its role in Ca2+-regulated ATP-Mg/Pi transport mediated by its transmembrane domain. While Ca2+-bound NTD could be crystallized, the apo state resisted extensive crystallization trials. Selenomethionine-labeled Ca2+-bound NTD crystals, which belonged to space group P6222 with one molecule per asymmetric unit, diffracted X-rays to 2.9 Å resolution.