Crystallization and preliminary X-ray data collection of the L27_PATJ-(L27N,L27C)_Pals1-L27_MALS tripartite complex

The L27 (LIN-2/LIN-7) domain is a protein-protein interaction module capable of assembling proteins into biologically important complexes. Pals1 contains two L27 domains: the first, L27N, interacts with PATJ, and the second, L27C, interacts with MALS, forming a tripartite complex that plays a crucial role in the establishment and maintenance of cell polarity. To provide a better understanding of the mechanism of assembly of this tripartite complex, four different L27_PATJ-(L27N,L27C)_Pals1-L27_MALS constructs were cloned, expressed, purified and crystallized. Crystals of tripartite complex 1 of L27_PATJ-(L27N,L27C)_Pals1-L27_MALS diffracted to 2.05 Å resolution. These crystals belonged to either space group P6₁22 or P6₅22, with unit-cell parameters a = b = 145.2, c = 202.5 Å. Assuming the presence of four molecules in the asymmetric unit, a Matthews coefficient of 2.69 Å³ Da^-1 was calculated, corresponding to a solvent content of 54.25%.