Structure of Citrobacter freundii L-methionine γ-­lyase

L-Methionine γ-lyase (MGL) is a pyridoxal 5′-phosphate (PLP) dependent enzyme that catalyzes γ-elimination of L-methionine. The crystal structure of MGL from Citrobacter freundii has been determined at 1.9 Å resolution. The spatial fold of the protein is similar to those of MGLs from Pseudomonas putida and Trichomonas vaginalis. The comparison of these structures revealed that there are differences in PLP-binding residues and positioning of the surrounding flexible loops.