Crystallization and preliminary crystallographic data of fructose-1,6-bisphosphatase from human muscle

The enzyme human muscle fructose-1,6-bisphosphatase, which plays a critical role in gluconeogenesis, has been crystallized in the presence of 2-propanol, polyethylene glycol and magnesium chloride at pH 7.5. The space group was determined to be P4₂2₁2, with unit-cell parameters a = b = 73.57, c = 146.50 Å, α = β = λ = 90° and one subunit in the asymmetric unit. A 99.6% complete data set to 2.04 Å has been collected at the National Synchrotron Light Source.