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A detailed description of the method of crystallographic refinement by simulated annealing is presented. To test the method, it has been applied to a 1.5 Å resolution X-ray structure of crambin. The dependence of the success of the simulated annealing protocol with respect to the temperature of the heating stage is discussed. Optimal success is achieved at relatively high temperatures. Regardless of the protocol used, the molecular-dynamics refined structure always yields an improved R factor compared with restrained least-squares refinement without manual re-fitting. The differences between the various refined structures and the corresponding electron density maps are discussed.
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