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Owing to the development of brilliant microfocus beamlines, rapid-readout detectors and sample changers, protein microcrystallography is rapidly becoming a popular technique for accessing structural information from complex biological samples. However, the method is time-consuming and labor-intensive and requires technical expertise to obtain high-resolution protein crystal structures. At SPring-8, an automated data-collection system named ZOO has been developed. This system enables faster data collection, facilitates advanced data-collection and data-processing techniques, and permits the collection of higher quality data. In this paper, the key features of the functionality put in place on the SPring-8 microbeam beamline BL32XU are described and the major advantages of this system are outlined. The ZOO system will be a major driving force in the evolution of the macromolecular crystallography beamlines at SPring-8.

Supporting information

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Link https://doi.org/10.5281/zenodo.1094808
Raw diffraction images of human muscarinic acetylcholine receptor.

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Link https://doi.org/10.5281/zenodo.1209098
Thermolysin dataset collected with the helical scheme implemented in HEBI in the ZOO system.

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Link https://doi.org/10.5281/zenodo.1209491
Thaumatin data collected with the SSROX scheme implemented in ZOO system.


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