Using a novel acquisition scheme for SAXS tensor tomography to obtain complete data for a millimeter-sized sample of human trabecular bone, we investigate and quantify the impact of the missing wedge problem on the reconstruction across real and reciprocal space.

A tomography reconstruction algorithm has been proposed that can be used for high-quality reconstruction of multi-gray-level material in `missing wedge' situations without prior knowledge of the gray values and manual segmentation.

An n-helix tilt scheme for cryo-electron tomography of pillar-shaped samples is described and simulated, allowing the entire pillar volume to be reconstructed as a single unit. Three related tilt schemes to enable the collection of the most isotropic information across all spatial frequencies are also evaluated.

Physical quantities in arbitrary dimensional space can be classified into 41 types using three antisymmetries within the framework of Clifford algebra.

STOPGAP, an open-source package for subtomogram averaging that is designed to provide users with fine control over each of the steps in the image-processing workflow, is described.

An environmental control system for in situ or operando nanotomography is presented. It allows controlling the sample temperature from room temperature to 850°C under controlled gas compositions, and a 3D isotropic resolution of sub-20 nm at a temperature of 600°C is demonstrated.

Based on the molecular architecture revealed by electron cryo-tomography, the mechanism of the bending motion of eukaryotic flagella/cilia is discussed.

A data-processing pipeline to solve structures from tomograms of protein nanocrystals is presented and validated using simulated crystals. The robustness of this workflow to radiation damage is assessed to investigate optimal data-collection strategies.

A tomography reconstruction algorithm has been proposed, which can achieve high-quality reconstructions of multiple grey phantoms from projection of a limited angle range and without prior knowledge of the grey values.

By using soft X-ray transmission microscopy with magnetic contrast (magnetic circular dichroism) it is possible to obtain valuable magnetic information from the studied systems. In this work, in a step further, a method to use magnetic soft X-ray transmission tomography as an ideal tool to reconstruct the three-dimensional magnetization configuration of arbitrary magnetic samples is presented.