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Acta Cryst. (2012). D68, 1010-1018
https://doi.org/10.1107/S0907444912019580

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Proline: Mother Nature's cryoprotectant applied to protein crystallography

T. A. Pemberton, B. R. Still, E. M. Christensen, H. Singh, D. Srivastava and J. J. Tanner

The amino acid L-proline is shown to be a good cryoprotectant for protein crystals. Four examples are provided; the range of proline used for cryoprotection is 2.0-3.0 M.

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