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3 citations found for McDonald, M.
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research papers
The Haas - Drenth - Wilson (HDW) (Haas et al., 1999) theoretical model was used to correlate osmotic second virial coefficient (B) values with solubility (S) values for equine serum albumin (ESA) and ovalbumin for corresponding solution conditions. The best fit from the theoretical model was compared to experimental S versus B data. B values were experimentally measured using static light scattering. Solubilities of ESA were estimated using a sitting drop method. When the experimental data for S versus B were plotted, an excellent fit for ESA was obtained according to the HDW model. The results showed that the coordination number (z) in the crystal lattice was 6, and the adjustable parameter (A) was 0.072. For ovalbumin, previously reported solubility data in aqueous ammonium sulfate solutions were utilized. The solubility data for ovalbumin were correlated with the measured B values obtained in our laboratory. The resulting best fit from the HDW model showed that z = 6 and A = 0.084.
research papers
The C-terminal His tag is resolved in the structures of the apoenzyme and complexes with AMP and pyrophosphate or a substrate analog of ATP, wherein it is found to interact with native residues at the dimer interface. In the complex structures, it partially occupies the native binding site for the substrate NaAD.
short communications
Compact, inexpensive, easily constructed sample holders for fiber diffraction allow humidity control, stretching and exposure to magnetic fields during fiber preparation, and primary containment for toxic and infectious biological materials.
