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L-Rhamnose isomerase (L-RhI) from B. halodurans has been purified and crystallized. The crystals of L-RhI belonged to the monoclinic space group P21, with unit-cell parameters a = 83.2, b = 164.9, c = 92.0 Å, β = 116.0°, and diffracted to 2.5 Å resolution.