Preparation of a crystallizable mRNA-binding fragment of Moorella thermoacetica elongation factor SelB

A C-terminal 30 kDa fragment of the selenocysteine-specific elongation factor SelB from M. thermoacetica has been recloned, expressed in E. coli and crystallized in a suitable form for X-ray analysis. The crystals diffract to 2.7 Å in an in-house X-ray source.