Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis

P450 MT2 (CYP121), a novel cytochrome P450 from M. tuberculosis that binds tightly to a range of azole-based antifungal drugs, has been expressed in E. coli, purified and crystallized. The crystals belong to either space group P6₁22 or P6₅22. Native crystals diffract to 1.6 Å, while a single-site Hg-derivative crystal diffracts to 2.5 Å resolution.

Crystallization and preliminary X-ray analysis of the hydroperoxidase I C-terminal domain from Escherichia coli

The C-terminal domain of catalase-peroxidase HPI (hydroperoxidase I) from E. coli has been crystallized.

Advantages of high-resolution phasing: MAD to atomic resolution

The structure of the endoglucanase A from Clostridium thermocellum CelA has been re-solved by three-wavelength MAD.

Physical estimation of triplet phases from two new proteins

Triplet phases have been obtained for three new proteins from three-beam diffraction. A brief discussion of strategies for the collection of triplet phases and their application in macromolecular crystallography is given.