Hydrogen-bond network and pH sensitivity in human transthyretin

The neutron crystal structure of human transthyretin is presented.

Crystal structure analysis, overexpression and refolding behaviour of a DING protein with single mutation

Crystals of a member of the DING protein family (HPBP) were obtained accidentally, and the structure was determined at 1.35 Å resolution. For further analysis, a system for preparation of HPBP was constructed and the structure of a prepared sample was confirmed by X-ray crystal structure analysis at 1.03 Å resolution.

Phase-diagram-guided method for growth of a large crystal of glycoside hydrolase family 45 inverting cellulase suitable for neutron structural analysis

The crystallization-phase-diagram-guided method is effective for growing large protein crystals for neutron protein crystallography.

Fundamental studies for the proton polarization technique in neutron protein crystallography

Fundamental trials to realise the proton polarization technique for detecting hydrogen with higher sensitivity in neutron protein crystallography are described.

Evaluation of performance for IBARAKI biological crystal diffractometer iBIX with new detectors

The time-of-flight neutron single-crystal diffractometer iBIX at the next-generation neutron source J-PARC has been upgraded and is available for user experiments on protein samples in particular. Neutron structure analysis of a standard protein sample was carried out in order to evaluate the performance of iBIX.