A new paradigm for macromolecular crystallography beamlines derived from high-pressure methodology and results

Macromolecular crystallography at high pressure (HPMX) is a mature technique. Shorter X-ray wavelengths increase data collection efficiency on cryocooled crystals. Extending applications and exploiting spin-off of HPMX will require dedicated synchrotron radiation beamlines based on a new paradigm.

Using lanthanoid complexes to phase large macromolecular assemblies

A lanthanoid complex, [Eu(DPA)₃]^3-, was used to obtain experimental phases at 4.0 Å resolution of PhTET1-12s, a large self-compartmentalized homo-dodecameric protease complex of 444 kDa.