Radiation damage in single-particle cryo-electron microscopy: effects of dose and dose rate

M. Karuppasamy, F. Karimi Nejadasl, M. Vulovic, A. J. Koster and R. B. G. Ravelli

The effects of dose and dose-rate were investigated for single-particle cryo-electron microscopy using stroboscopic data collection. A dose-rate effect was observed favoring lower flux densities.

Keywords: single-particle cryo-electron microscopy; radiation damage; dose; dose-rate effect; macromolecular X-ray crystallography.

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