The structure of Enterococcus faecalis thymidylate synthase provides clues about folate bacterial metabolism

The crystal structure of thymidylate synthase from E. faecalis was obtained as a native complex with 5-formyltetrahydrofolate (5-FTHF). The structure supports the role of 5-FTHF, which is naturally present in the bacterial cell, as a storage form of the folate metabolite and as an enzyme regulator.