crystallization communications
Biosynthetic alanine racemase from P. aeruginosa PAO1 was expressed in E. coli and purified. The purified enzyme was crystallized and preliminary X-ray crystallographic analysis was performed.
crystallization communications
McbB, a multifunctional enzyme responsible for catalysing Pictet–Spengler cyclization, decarboxylation and oxidation reactions in the biosynthesis of β-carboline, was expressed and crystallized. The crystals belonged to the monoclinic space group P21, with unit-cell parameters a = 66.06, b = 85.48, c = 106.19 Å, α = 90.00, β = 106.77, γ = 90.00°.