Low-resolution structure determination of Na⁺-translocating NADH:ubiquinone oxidoreductase from Vibrio cholerae by ab initio phasing and electron microscopy

A 16 Å resolution molecular envelope of Na⁺-translocating NADH:ubiquinone oxidoreductase was determined by connectivity-based ab initio phasing. The low-resolution structure of this multi-subunit membrane complex was confirmed by negative-stain electron microscopy.