Structure of a domain-opened mutant (R121D) of the human lactoferrin N-lobe refined from a merohedrally twinned crystal form

The structure of the R121D mutant of the N-lobe half-molecule of human lactoferrin has been determined and refined at 3.0 Å resolution using data from a merohedrally twinned crystal form. The mutant, in its iron-free form, has a domain-opened structure that matches that of the N-lobe of full-length human lactoferrin.