Crystallization and preliminary X-ray diffraction studies of a surface mutant of the middle domain of PB2 from human influenza A (H1N1) virus

A double mutation (P453H and I471T) improved the solubility of the middle domain of PB2 from influenza A virus and the shape of the crystals that were obtained. A small crystal was used to test the diffraction and a preliminary X-ray diffraction data set was collected to 2.30 Å resolution.