Crystallization and preliminary X-ray crystallographic analysis of bacterial tRNA^Sec in complex with seryl-tRNA synthetase

Bacterial selenocysteine tRNA was crystallized as the heterologous complex with archaeal seryl-tRNA synthetase. X-ray diffraction was improved by introducing point mutations and heavy-atom labeling, and a 3.2 Å diffraction data set for phase determination was obtained from a platinum-labeled crystal.

Flexibility of the P-loop of Pim-1 kinase: observation of a novel conformation induced by interaction with an inhibitor

The structures of Pim1 kinase in complex with in silico screening hits and several subsequently optimized inhibitors, are reported.

Crystallization and preliminary X-ray crystallographic analysis of Aquifex aeolicus SelA, a bacterial selenocysteine synthase

The bacterial selenocysteine synthase SelA from Aquifex aeolicus was crystallized and the diffraction resolution was improved by lysine-residue methylation, truncation of N-terminal region (ΔN), and Lys-to-Ala point mutations. Phases were determined by using a selenomethionine-substituted crystal of the ΔN mutant.

Structure of the Rho-specific guanine nucleotide-exchange factor Xpln

The Xpln crystal structure provides structural insights into Rho GTPase binding.