Purification, crystallization and preliminary X-ray crystallographic analysis of squid heavy meromyosin

In various muscles, the two heads of myosin together with part of its α-helical coiled coil make up the minimal unit that is regulated. Reported here, for the first time, is the growth of three-dimensional crystals of such a heavy meromyosin, purified from squid, and their X-ray diffraction to near-atomic resolution (up to ∼5 Å).