Using high-throughput in situ plate screening to evaluate the effect of dehydration on protein crystals

A novel high-throughput in situ plate-screening procedure is used to assess the effect of dehydration on a membrane-associated protein. In this case the dehydration improved the diffraction quality of the crystal.

S-Adenosyl-S-carboxymethyl-L-homocysteine: a novel cofactor found in the putative tRNA-modifying enzyme CmoA

The putative methyltransferase CmoA is involved in the nucleoside modification of transfer RNA. X-ray crystallography and mass spectrometry are used to show that it contains a novel SAM derivative, S-adenosyl-S-carboxymethyl-L-homocysteine, in which the donor methyl group is replaced by a carboxymethyl group.

Clustering procedures for the optimal selection of data sets from multiple crystals in macromolecular crystallography

A systematic approach to the scaling and merging of data from multiple crystals in macromolecular crystallography is introduced and explained.