In situ macromolecular crystallography using microbeams

D. Axford, R. L. Owen, J. Aishima, J. Foadi, A. W. Morgan, J. I. Robinson, J. E. Nettleship, R. J. Owens, I. Moraes, E. E. Fry, J. M. Grimes, K. Harlos, A. Kotecha, J. Ren, G. Sutton, T. S. Walter, D. I. Stuart and G. Evans

A sample environment for mounting crystallization trays has been developed on the microfocus beamline I24 at Diamond Light Source. The technical developments and several case studies are described.

Keywords: in situ diffraction; microfocus; microbeams; high throughput; room temperature; viruses; membrane proteins; synchrotron radiation.

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Outrunning free radicals in room-temperature macromolecular crystallography

R. L. Owen, D. Axford, J. E. Nettleship, R. J. Owens, J. I. Robinson, A. W. Morgan, A. S. Doré, G. Lebon, C. G. Tate, E. E. Fry, J. Ren, D. I. Stuart and G. Evans

A systematic increase in lifetime is observed in room-temperature protein and virus crystals through the use of reduced exposure times and a fast detector.

Keywords: radiation damage; room temperature; dose rate; free radicals.

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Nearest-cell: a fast and easy tool for locating crystal matches in the PDB

V. Ramraj, G. Evans, J. M. Diprose and R. M. Esnouf

A fast and easy tool to locate unit-cell matches in the PDB is described.

Keywords: Nearest-cell; crystal matches; Protein Data Bank.

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