In situ macromolecular crystallography using microbeams

D. Axford, R. L. Owen, J. Aishima, J. Foadi, A. W. Morgan, J. I. Robinson, J. E. Nettleship, R. J. Owens, I. Moraes, E. E. Fry, J. M. Grimes, K. Harlos, A. Kotecha, J. Ren, G. Sutton, T. S. Walter, D. I. Stuart and G. Evans

A sample environment for mounting crystallization trays has been developed on the microfocus beamline I24 at Diamond Light Source. The technical developments and several case studies are described.

Keywords: in situ diffraction; microfocus; microbeams; high throughput; room temperature; viruses; membrane proteins; synchrotron radiation.

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