Use of differential scanning fluorimetry to optimize the purification and crystallization of PLP-dependent enzymes

T. W. Geders, K. Gustafson and B. C. Finzel

The utility of differential scanning fluorimetry for homogeneity assessment and crystallization improvement of PLP-dependent enzymes is demonstrated using the potential drug target BioA from M. tuberculosis.

Keywords: differential scanning fluorimetry; ThermoFluor; crystallization optimization; pyridoxal 5'-phosphate; UV-Vis spectroscopy.

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