Cloning, expression, purification and preliminary X-ray analysis of EstN2, a novel archaeal α/β-hydrolase from Candidatus Nitrososphaera gargensis

The chemolithoautotrophic nitrifying thaumarchaeon Ca. Nitrososphaera gargensis with unusual metabolic pathways was enriched by cultivation over six years. A novel α/β-hydrolase was identified, isolated and crystallized for further functional analysis.