Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the bovine CD8αα–BoLA-2*02201 complex

The crystallization and preliminary X-ray diffraction analysis of a complex between bovine CD8αα and the bovine MHC class I molecule (BoLA-I, BoLA-2*02201) at 1.7 Å resolution revealed that the crystal belonged to space group P2₁, with unit-cell parameters a = 53.9, b = 103.8, c = 61.8 Å, α = γ = 90, β = 96°, and contained one complex in the asymmetric unit. The Matthews coefficient and solvent content were calculated as 2.41 ų Da⁻¹ and 48.9%, respectively.

Cloning, purification, crystallization and preliminary X-ray studies of the putative type VI secretion immunity protein Tli5 (PA5088) from Pseudomonas aeruginosa

Here, the expression, purification, crystallization and preliminary crystallographic analysis of putative protein PA5088 from Pseudomonas aeruginosa are reported.