Expression, crystallization and preliminary X-ray crystallographic analysis of alanine racemase from Acinetobacter baumannii OXA-23

Alanine racemase (ALR) catalyzes the racemization of L-alanine to D-alanine with pyridoxal 5'-phosphate (PLP) as a cofactor. The alr gene from A. baumannii OXA-23 was cloned and the protein was expressed, purified and crystallized. A preliminary X-ray crystallographic analysis of the ALR crystal was performed.

Expression, crystallization and preliminary X-ray crystallographic analysis of peptide deformylase from Campylobacter jejuni

Peptide deformylase catalyzes the deformylation of N-formylated methionine in newly synthesized polypeptides in prokaryotes and some eukaryotic organelles. The synthetic codon-optimized def gene from C. jejuni was cloned and the protein expressed, purified and crystallized. A preliminary X-ray crystallography analysis of the C. jejuni peptide deformylase crystals was performed.