Crystallization and preliminary X-ray crystallographic studies of DnaJ from Streptococcus pneumoniae

DnaJ from Streptococcus pneumoniae (SpDnaJ) is involved in the infectious disease process and is being developed as a potential vaccine to prevent bacterial infection. Here the expression, purification, crystallization and preliminary crystallographic analysis of SpDnaJ are reported.

Purification, crystallization and preliminary X-ray crystallographic study of the tRNA pseudouridine synthase TruB from Streptococcus pneumoniae

The RNA pseudouridine synthase TruB catalyses the isomerization of uridine to pseudouridine at residue 55 of elongator tRNAs. Here, the expression, purification, crystallization and preliminary crystallographic analysis of TruB from Streptococcus pneumoniae are reported.

Cloning, purification, crystallization and preliminary X-ray studies of the putative transcriptional regulator SPD0280 from Streptococcus pneumoniae D39

The cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the putative transcriptional regulator SPD0280 from S. pneumoniae D39 are described.

Recombinant production, crystallization and preliminary structural characterization of Schistosoma japonicum profilin

Profilin from the helminthic parasite S. japonicum was expressed, purified and crystallized. The protein was found to be folded and to possess the expected secondary-structure content as determined by circular-dichroism spectroscopy.