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DnaJ from Streptococcus pneumoniae (SpDnaJ) is involved in the infectious disease process and is being developed as a potential vaccine to prevent bacterial infection. Here the expression, purification, crystallization and preliminary crystallographic analysis of SpDnaJ are reported.

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The crystal of E. coli 23S rRNA methyltransferase RlmM in this study belongs to a new space group (P21) and is different from the known P31 or P3121 space groups.

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The RNA pseudouridine synthase TruB catalyses the isomerization of uridine to pseudouridine at residue 55 of elongator tRNAs. Here, the expression, purification, crystallization and preliminary crystallographic analysis of TruB from Streptococcus pneumoniae are reported.

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The cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the putative transcriptional regulator SPD0280 from S. pneumoniae D39 are described.
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