Cloning, expression, purification and preliminary X-­ray analysis of a putative metagenome-derived lipase

A putative lipase, LipS, from the soil metagenome of an environmental sample taken at the Biocenter Klein Flottbek, Hamburg, Germany was cloned, expressed and purified. Two different constructs of the protein were crystallized in a search for well diffracting crystals. Preliminary X-ray analyses indicated that crystals in space groups P4₂2₁2 and P4 diffracted X-ray radiation to 2.8 and 2.0 Å resolution, respectively.