Expression, high-pressure refolding, purification, crystallization and preliminary X-ray analysis of a novel single-strand-specific 3'-5' exonuclease PhoExo I from Pyrococcus horikoshii OT3

A single-strand-specific 3'-5' exonuclease, PhoExo I, from the hyperthermophilic archaeon P. horikoshii OT3 was produced as inclusion bodies in E. coli cells, solubilized by the high-pressure refolding method, purified and crystallized. The crystal of PhoExo I diffracted X-rays to 1.52 Å resolution.