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The expression, purification, crystallization, preliminary X-ray crystallographic and cryo-EM analysis of the bifunctional enzyme fucokinase/L-fucose-1-P-guanylyltransferase are reported. A diffraction data set was collected to 3.7 Å resolution from the full-length native protein crystal using synchrotron X-rays. The preliminary cryo-EM image showed two parallel disc-shaped objects with a diameter of 10 nm.
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