Expression, crystallization and preliminary X-ray crystallographic analysis of D-alanine-D-alanine ligase from OXA-23-producing Acinetobacter baumannii K0420859

OXA-23-producing A. baumannii K0420859 can resist the action of carbapenem, and causes bacteraemia, pneumonia, other respiratory-tract and urinary-tract infections in humans. As a novel target for an antibacterial drug against A. baumannii OXA-23, D-alanine-D-alanine ligase from A. baumannii was purified and crystallized, and a primary X-ray crystallographic analysis was performed.

Crystallization and preliminary X-ray crystallographic analysis of the XoGroEL chaperonin from Xanthomonas oryzae pv. oryzae

The XoGroEL gene (XOO_4288) was cloned from X. oryzae pv. oryzae and the protein was expressed, purified and crystallized. A preliminary X-ray crystallographic analysis of an XoGroEL crystal was performed.

Expression, crystallization and preliminary X-ray crystallographic analysis of DNA-directed RNA polymerase subunit L from Thermococcus onnurineus NA1

The TON-0309 gene encoding DNA-directed RNA polymerase subunit L (ToRNAP_L) from T. onnurineus NA1 was cloned and the protein was expressed in E. coli, purified and crystallized. A preliminary X-ray crystallographic analysis of a ToRNAP_L crystal was performed.

Expression, crystallization and preliminary X-ray crystallographic analysis of cystathionine β-lyase from Acinetobacter baumannii OXA-23

Cystathionine β-lyase from multidrug-resistant A. baumannii OXA-23 (AbCBL), an enzyme involved in the methionine-metabolism pathway and a novel antibacterial drug target, was cloned, expressed, purified and crystallized. Preliminary X-ray crystallography was performed to analyse the AbCBL crystal.