Purification, crystallization and preliminary X-ray diffraction analysis of inner membrane complex (IMC) subcompartment protein 1 (ISP1) from Toxoplasma gondii

To characterize the ISP family of proteins present in apicomplexan parasites, ISP1 from T. gondii was expressed, purified and crystallized. Two crystal forms (cubic and orthorhombic) were analyzed by X-ray diffraction and data were processed to 2.05 and 2.1 Å resolution, respectively.

Purification, crystallization and X-ray diffraction analysis of Trypanosoma congolense insect-stage surface antigen (TcCISSA)

A novel stage-specific surface protein, T. congolense insect-stage surface antigen (TcCISSA), from the African trypanosome T. congolense was recently identified by mass-spectrometric differential protein-expression analysis. To gain structure-function insight into this protein, the extracellular domain of TcCISSA was expressed, purified and crystallized and X-ray diffraction data were collected and processed to 2.7 Å resolution.