Crystallization, X-ray characterization and selenomethionine phasing of Mlc1p bound to IQ motifs from myosin V

Mlc1p is a myosin light chain that binds to IQ motifs of a class V myosin (Myo2p) and an IQGAP-like protein (Iqg1p), playing a role in cytokinesis and organelle transport in S. cerevisiae. Complexes of Mlc1p with IQ motifs 2, 3, 4, and 2,3 of Myo2p were crystallized and their structures determined.