Purification, crystallization and preliminary structural studies of dTDP-4-keto-6-deoxy-glucose-5-epimerase (EvaD) from Amycolatopsis orientalis, the fourth enzyme in the dTDP-L-epivancosamine biosynthetic pathway

Vancomycin is the last line of defence in the treatment of Gram-positive infection. It is a complex molecule composed of carbohydrate and cyclized peptides. The crystallization of dTDP-3-amino-4-keto 2,3,6-trideoxy-3-C-methyl-glucose-5-epimerase, an enzyme involved in the synthesis of the carbohydrate portion of the compound, is reported.