Crystallization and preliminary X-ray crystallographic studies of HLA-A*1101 complexed with an HIV-1 decapeptide

The first soluble recombinant class I HLA-A*1101 molecule has been expressed and refolded in vitro with β2-microglobulin and a Nef decapeptide. The complex was crystallized and X-ray diffraction data to 2.2 Å resolution were collected.

Parallel cloning, expression, purification and crystallization of human proteins for structural genomics

Insights are gained into high-throughput target selection, cloning, expression and crystallization of human genomic proteins. For example, there was a marked difference seen bewteen the ease of expression of proteins of molecular weight (MW) < 25 kDa (93% success), compared with proteins > 25 kDa MW, which were less easy to express (61% success).