Protein expression, characterization, crystallization and preliminary X-ray crystallographic analysis of a Fic protein from Clostridium difficile

A Fic protein from the pathogenic bacterium C. difficile R20291 has been cloned, expressed and purified. The purified protein was characterized by mass spectrometry and circular-dichroism spectroscopy, and was crystallized. In addition, a diffraction data set was collected and processed to 1.68 Å resolution.