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The purification and crystallization of the isolated toxin VapC (MvpT) from the VapBC toxin–antitoxin complex of the pathogen S. flexneri are reported. The crystals belonged to space group H3 and diffracted to 1.9 Å resolution.

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An automated spreadsheet-based solution for macromolecular crystallization screening is presented that allows easy formulation and optimization of crystal screens via multiple approaches, including incomplete-factorial and grid screening.
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