Cloning, expression, purification, crystallization and X-ray crystallographic analysis of the (S)-3-hydroxybutyryl-CoA dehydrogenase PaaH1 from Ralstonia eutropha H16

The (S)-3-hydroxybutyryl-CoA dehydrogenase PaaH1 from R. eutropha (RePaaH1), an enzyme involved in n-butanol biosynthesis, has been crystallized, and X-ray diffraction data have been collected and analyzed.